Alkylphenol Ethoxylate (APE), ELISA, 96-test

Immunoassay for the quantitative and sensitive detection of Alkylphenol Ethoxylate (APE).

Kit Principle

Competitive Reaction: The test is based on the recognition of APE by specific monoclonal antibodies. APE present in the sample and an APE-enzyme conjugate (i.e. APE labeled with a coloring enzyme) are premixed and added into each well of a microplate, and allowed to compete for limited number of binding sites of specific antibodies immobilized on the surface of the wells. When the APE concentration is higher relative to the enzyme conjugate, the APE will predominantly bind the antibody and vice versa.

Chromogenic Reaction: Unbound APE and excess antigen-enzyme conjugates are washed out. The chromogenic substrate is then added to develop color in conjunction with the enzyme conjugate. The amount of APE-enzyme conjugate remaining bound to the antibody will determine the color intensity. The higher APE concentration in sample, for example, leads to less antigen-enzyme conjugate bound to the antibody binding sites in a microplate well, generating a lighter color, i.e. lower absorbance.

Quantitative Analysis: The standard curve, a dose-response curve obtained from known concentrations of APE standards, is determined from the absorbance at 450nm. The APE concentration in each sample is accurately calculated by interpolation using the absorbance intensity obtained from the standard curve.

Features: -The monoclonal antibody binds exclusively with Alkylphenol Ethoxylate (APE)and Alkylphenol ethoxy acetic acid, and does not cross-react with othersurfactants or compounds of similar structure. -The detection range is between 0.02mg/L and 1.0mg/L. A simple concentration protocol based on solid phase extraction is available to determine much lower concentration. -With ease of handling, the total time for measurement is only 2.5 hours. -The kit, a 96-well microplate format, enables simultaneous measurement of multiple samples at more reasonable cost.

PN 520041

Made to Order - Minimum Order Quantity 5 Kits

Format: 96-Well Plate ELISA