Domoic Acid (ASP) Alert™ Test Strip Shellfish; 5-Test

Sensoreal ASP Alert™ is a rapid immunochromatographic lateral flow designed for the qualitative and semi-quantitative screening of Domoic Acid (the toxin associated with amnesic shellfish poisoning (ASP) in bivalve mollusks (mussels, oysters, scallops, cockles, razor and hard-shell clams?). The assay uses a competitive immunoassay format. Domoic Acid is extracted from homogenized shellfish tissue using a 100% methanol solution. The extract is then diluted and incubated with a lyophilized antibody–gold nanoparticle conjugate. If Domoic Acid is present in the sample, it binds to the conjugate antibodies, reducing the amount of free antibody–gold complex available. The mixture is then applied to the lateral flow cassette and migrates across a membrane containing immobilized Domoic Acid-protein at the test line. A control line must appear for the test to be considered valid, confirming proper sample migration and assay performance. Test-line intensity is inversely proportional to the toxin concentration: as the concentration of Domoic Acid, the intensity of the test line decreases. A negative result is indicated when the test line color is equal to or greater than the intensity of the control line, indicating that the assay did not detect Domoic Acid at or above the method detection limit. Conversely, a test line with color below that of the control line intensity indicates a presumptive positive result. Presumptive positive samples may be confirmed using ELISA, HPLC, or another appropriate confirmatory analytical method.

Alert™ Test Strip Shellfish Filter Pack accessory pack is available for in-field shellfish testing.

PN 520063

Format Dipstick Test

PN 520085

Format Filter Field Accessory Pack