ABRAXIS® Anatoxin-a (VFDF), ELISA, 96-test

The Anatoxin-a ELISA Plate Kit is an immunoassay for the quantitative and sensitive screening of Anatoxin-a in water samples. This test is suitable for the quantitative and/or qualitative screening of Anatoxin-a in drinking and recreational water samples. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.

The test is a direct competitive ELISA based on the recognition of Anatoxin-a by a monoclonal antibody. Anatoxin-a, when present in a sample, and an Anatoxin-a-enzyme conjugate compete for the binding sites of mouse anti-Anatoxin-a antibodies in solution. The Anatoxin-a antibodies are then bound by a second antibody (anti-mouse) immobilized on the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Anatoxin-a present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

Learn more about our Algal Toxin Proficiency Testing Program

PN 520060

Format: 96-Well Plate ELISA