{"text_format":"1","workbook":"xlsx","n_sheets":6,"sheets":["INSTRUCTIONS","4 Standards","5 Standards","6 Standards","7 Standards","8 Standards"],"not_rendered":{"charts":5,"images":1,"macros":0,"external_links":0,"defined_names":140,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"INSTRUCTIONS","index":0,"n_rows":8,"n_cols":7,"rows_kept":8,"cut":false,"hidden":false,"merged":[[0,0,5,10],[6,0,6,10],[7,0,7,5],[7,6,7,10]]}
[]
[]
[]
[]
[]
[]
["For instructions for use of the solver, please see this page."]
["For questions or technical issues, please email ",null,null,null,null,null,"support.abraxis@us.goldstandarddiagnostics.com"]

{"sheet":"4 Standards","index":1,"n_rows":143,"n_cols":19,"rows_kept":143,"cut":false,"hidden":false,"merged":[[133,7,134,9],[135,7,136,9],[137,7,138,9],[139,7,140,9],[141,7,142,9],[99,7,100,9],[101,7,102,9],[103,7,104,9],[105,7,106,9],[95,7,96,9],[87,7,88,9],[89,7,90,9],[91,7,92,9],[93,7,94,9],[97,7,98,9],[79,7,80,9],[81,7,82,9],[129,7,130,9],[131,7,132,9],[109,7,110,9],[111,7,112,9],[113,7,114,9],[115,7,116,9],[117,7,118,9],[119,7,120,9],[121,7,122,9],[123,7,124,9],[125,7,126,9],[127,7,128,9],[107,7,108,9],[85,7,86,9],[83,7,84,9],[71,7,72,9],[49,7,50,9],[51,7,52,9],[53,7,54,9],[55,7,56,9],[57,7,58,9],[59,7,60,9],[61,7,62,9],[63,7,64,9],[65,7,66,9],[67,7,68,9],[69,7,70,9],[73,7,74,9],[75,7,76,9],[77,7,78,9],[47,7,48,9],[14,15,14,24],[16,15,16,24],[18,1,18,7],[33,1,33,9],[34,7,34,9],[35,7,36,9],[37,7,38,9],[39,7,40,9],[41,7,42,9],[43,7,44,9],[45,7,46,9],[13,15,13,24],[1,1,1,5],[1,15,1,24],[3,15,3,24],[5,15,5,24],[6,15,6,24],[7,15,7,24],[8,15,8,24],[9,15,9,24],[11,15,11,24],[12,15,12,24],[3,1,3,4]]}
[]
[null,"** USER MUST ONLY INPUT VALUES IN GREY-COLORED CELLS **",null,null,null,null,"Kit Lot #",null,null,null,null,null,null,null,null,"Before you start:"]
[]
[null,"Enter the absorbance values of each standard into the grey boxes",null,null,null,null,null,null,null,null,null,null,null,null,null,"Check that \"solver\" is installed"]
[null,"Std 0","Std 1","Std 2","Std 3"]
[null,2.149,1.7749999999999999,1.242,0.48899999999999999,null,null,null,null,null,null,null,5.8999999999999997E-2,null,null,"Input OD values for standards (Fig 4 in protocol)"]
[null,2.0720000000000001,1.804,1.1930000000000001,0.48199999999999998,null,null,null,null,null,null,null,6.0999999999999999E-2,null,null,"Select solver  (Target Cell: $e$32 - Cell Changes: $c$35:$c$38 - Optimize: Minimize - GRG Nonlinear )"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"\"Solve\""]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Check graph and parametres (A>0.7; D<0.1; "]
[]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Calculate MC TADDA-concentration in samples in wells: "]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Input average OD of unknown samples in cell c48 (sample 1) , c49 (sample 2)…."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Calculated ELISA MCTADDA concentration are given automatically in cell d48 (sample 1), c49 (sample 2),…."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Sample concentrations should only be used from datapoints that are within the valid working range of the standard curve"]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"If OD is outsite the Ic20 to Ic80 range, dilution sholud be adjusted and the samples assayed again"]
[]
[null,"Enter the standard concentrations of each standard into the grey boxes"]
[null,"Standards (ng/ml)","OD (averaged)","Predicted values","Residual Squares","B/Bo","Std Dev","%CV"]
[null,0,{"$f":"(B6+B7)/2","v":2.1105},{"$f":"($C$28-$C$31)/(1+(B21/$C$30)^$C$29)+$C$31","v":2.1012199223423793},{"$f":"(C21-D21)^2","v":8.6119841331471511E-5},null,{"$f":"STDEV(B6,B7)","v":5.4447222151364126E-2},{"$f":"(G21/C21)*100","v":2.5798257356723111}]
[null,0.15,{"$f":"(C6+C7)/2","v":1.7894999999999999},{"$f":"($C$28-$C$31)/(1+(B22/$C$30)^$C$29)+$C$31","v":1.8099070880700161},4.1644924349739884E-4,{"$f":"C22/C$21","v":0.84790334044065374},{"$f":"STDEV(C6,C7)","v":2.0506096654409976E-2},1.1459120790393951,null,null,null,null,null,null,null,null,"OD","MCTADDA-conc","X=C x ((A-Y)/(Y-D))^(1/B)"]
[null,0.4,{"$f":"(D6+D7)/2","v":1.2175},{"$f":"($C$28-$C$31)/(1+(B23/$C$30)^$C$29)+$C$31","v":1.1969170366462512},4.2365838042176905E-4,{"$f":"C23/C$21","v":0.57687751717602465},{"$f":"STDEV(D6,D7)","v":3.4648232278140782E-2},2.8458507004633087,null,null,null,null,null,null,null,"Ic20",{"$f":"$C$31+($C$28-$C$31)*20/100","v":0.42024398446847583},{"$f":"C30*((C28-Q23)/(Q23-C31))^(1/C29)","v":1.1512775802703727}]
[null,1,{"$f":"(E6+E7)/2","v":0.48549999999999999},{"$f":"($C$28-$C$31)/(1+(B24/$C$30)^$C$29)+$C$31","v":0.49988798537032963},2.0701412301681998E-4,{"$f":"C24/C$21","v":0.23004027481639422},{"$f":"STDEV(E6,E7)","v":4.9497474683058368E-3},1.0195154414636123,null,null,null,null,null,null,null,"Ic80",{"$f":"$C$31+($C$28-$C$31)*80/100","v":1.6809759378739033},{"$f":"C30*((C28-Q24)/(Q24-C31))^(1/C29)","v":0.19833282905229538}]
[null,"Sum Of Squares",null,null,{"$f":"SUM(E21:E24)","v":1.1332415882674594E-3}]
[]
[null,"Parameter (Y=(A-D)/(1+(X/C)^B)+D)",null,null,"R-Squared"]
[null,"A",2.1012199223423793,"(Max.)",{"$f":"(CORREL(C21:C24,D21:D24))^2","v":0.99928220927559885}]
[null,"B",1.5765160195861767,"(Slope)"]
[null,"C",0.47784530919483137,"(IC50)"]
[null,"D",0,"(Min.)"]
[]
[]
[null,"Enter descriptions and absorbance values of each sample and control into the grey boxes"]
[null,"Sample/Control","Absorbances","Std Dev","%CV","Sample Concentration (ng/ml)","Sample Concentration Avg (ng/ml)","Comments"]
[null,"Control",0.49099999999999999,null,null,{"$f":"IF(ISBLANK(C36),\"\",IF(C36<=$C$31,\"Invalid\",IF(C36>=$C$28,\"Invalid\",$C$30*(($C$28-C36)/(C36-$C$31))^(1/$C$29))))","v":1.0150018052926144},null,{"$f":"IF(AND(ISBLANK(C36),ISBLANK(C37)),\"\",IF(AVERAGE($C36:$C37)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C36:$C37)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,"Control",0.51100000000000001,{"$f":"IF(OR(ISBLANK(C36),ISBLANK(C37)),\"\",STDEV(C36,C37))","v":1.4142135623730963E-2},{"$f":"IF(ISTEXT(D37),\"\",D37/((C36+C37)/2)*100)","v":2.8227815616229468},{"$f":"IF(ISBLANK(C37),\"\",IF(C37<=$C$31,\"Invalid\",IF(C37>=$C$28,\"Invalid\",$C$30*(($C$28-C37)/(C37-$C$31))^(1/$C$29))))","v":0.98180494413895836},{"$f":"IF(AND(ISBLANK(C36),ISBLANK(C37)),\"\",IF(OR(ISBLANK(C37),ISBLANK(C36)),AVERAGE(F36:F37),IF(OR(ISTEXT(F36),ISTEXT(F37)),\"Invalid\",AVERAGE(F36:F37))))","v":0.99840337471578633}]
[null,"Sample 1",1.23,null,null,0.38395653842149435,null,{"$f":"IF(AND(ISBLANK(C38),ISBLANK(C39)),\"\",IF(AVERAGE($C38:$C39)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C38:$C39)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,"Sample 1",1.25,{"$f":"IF(OR(ISBLANK(C38),ISBLANK(C39)),\"\",STDEV(C38,C39))","v":1.4142135623730963E-2},{"$f":"IF(ISTEXT(D39),\"\",D39/((C38+C39)/2)*100)","v":1.1404948083654003},0.37449077569213574,{"$f":"IF(AND(ISBLANK(C38),ISBLANK(C39)),\"\",IF(OR(ISBLANK(C39),ISBLANK(C38)),AVERAGE(F38:F39),IF(OR(ISTEXT(F38),ISTEXT(F39)),\"Invalid\",AVERAGE(F38:F39))))","v":0.37922365705681504}]
[null,"Sample 2",1.45,null,null,0.28759118206870543,null,{"$f":"IF(AND(ISBLANK(C40),ISBLANK(C41)),\"\",IF(AVERAGE($C40:$C41)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C40:$C41)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,"Sample 2",1.405,{"$f":"IF(OR(ISBLANK(C40),ISBLANK(C41)),\"\",STDEV(C40,C41))","v":3.1819805153394588E-2},{"$f":"IF(ISTEXT(D41),\"\",D41/((C40+C41)/2)*100)","v":2.2290581543533863},0.30610275079132265,{"$f":"IF(AND(ISBLANK(C40),ISBLANK(C41)),\"\",IF(OR(ISBLANK(C41),ISBLANK(C40)),AVERAGE(F40:F41),IF(OR(ISTEXT(F40),ISTEXT(F41)),\"Invalid\",AVERAGE(F40:F41))))","v":0.29684696643001407}]
[null,"Sample 3",1.63,null,null,0.21747886344651263,null,{"$f":"IF(AND(ISBLANK(C42),ISBLANK(C43)),\"\",IF(AVERAGE($C42:$C43)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C42:$C43)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,"Sample 3",1.65,{"$f":"IF(OR(ISBLANK(C42),ISBLANK(C43)),\"\",STDEV(C42,C43))","v":1.4142135623730963E-2},{"$f":"IF(ISTEXT(D43),\"\",D43/((C42+C43)/2)*100)","v":0.86232534291042462},0.20994718868766807,{"$f":"IF(AND(ISBLANK(C42),ISBLANK(C43)),\"\",IF(OR(ISBLANK(C43),ISBLANK(C42)),AVERAGE(F42:F43),IF(OR(ISTEXT(F42),ISTEXT(F43)),\"Invalid\",AVERAGE(F42:F43))))","v":0.21371302606709036}]
[null,"Sample 4",0.52,null,null,0.96750245481212382,null,{"$f":"IF(AND(ISBLANK(C44),ISBLANK(C45)),\"\",IF(AVERAGE($C44:$C45)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C44:$C45)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,"Sample 4",0.51,{"$f":"IF(OR(ISBLANK(C44),ISBLANK(C45)),\"\",STDEV(C44,C45))","v":7.0710678118654814E-3},{"$f":"IF(ISTEXT(D45),\"\",D45/((C44+C45)/2)*100)","v":1.3730228760903846},0.98341768811157038,{"$f":"IF(AND(ISBLANK(C44),ISBLANK(C45)),\"\",IF(OR(ISBLANK(C45),ISBLANK(C44)),AVERAGE(F44:F45),IF(OR(ISTEXT(F44),ISTEXT(F45)),\"Invalid\",AVERAGE(F44:F45))))","v":0.9754600714618471}]
[null,null,2.2999999999999998,null,null,"Invalid",null,{"$f":"IF(AND(ISBLANK(C46),ISBLANK(C47)),\"\",IF(AVERAGE($C46:$C47)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C46:$C47)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":"LESS than Standard 1, outside of quantifiable range"}]
[null,null,2.2999999999999998,{"$f":"IF(OR(ISBLANK(C46),ISBLANK(C47)),\"\",STDEV(C46,C47))","v":0},{"$f":"IF(ISTEXT(D47),\"\",D47/((C46+C47)/2)*100)","v":0},"Invalid",{"$f":"IF(AND(ISBLANK(C46),ISBLANK(C47)),\"\",IF(OR(ISBLANK(C47),ISBLANK(C46)),AVERAGE(F46:F47),IF(OR(ISTEXT(F46),ISTEXT(F47)),\"Invalid\",AVERAGE(F46:F47))))","v":"Invalid"}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C48),ISBLANK(C49)),\"\",IF(AVERAGE($C48:$C49)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C48:$C49)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C48),ISBLANK(C49)),\"\",STDEV(C48,C49))","v":""},{"$f":"IF(ISTEXT(D49),\"\",D49/((C48+C49)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C48),ISBLANK(C49)),\"\",IF(OR(ISBLANK(C49),ISBLANK(C48)),AVERAGE(F48:F49),IF(OR(ISTEXT(F48),ISTEXT(F49)),\"Invalid\",AVERAGE(F48:F49))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C50),ISBLANK(C51)),\"\",IF(AVERAGE($C50:$C51)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C50:$C51)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C50),ISBLANK(C51)),\"\",STDEV(C50,C51))","v":""},{"$f":"IF(ISTEXT(D51),\"\",D51/((C50+C51)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C50),ISBLANK(C51)),\"\",IF(OR(ISBLANK(C51),ISBLANK(C50)),AVERAGE(F50:F51),IF(OR(ISTEXT(F50),ISTEXT(F51)),\"Invalid\",AVERAGE(F50:F51))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C52),\"\",IF(C52<=$C$31,\"Invalid\",IF(C52>=$C$28,\"Invalid\",$C$30*(($C$28-C52)/(C52-$C$31))^(1/$C$29))))","v":""},null,{"$f":"IF(AND(ISBLANK(C52),ISBLANK(C53)),\"\",IF(AVERAGE($C52:$C53)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C52:$C53)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C52),ISBLANK(C53)),\"\",STDEV(C52,C53))","v":""},{"$f":"IF(ISTEXT(D53),\"\",D53/((C52+C53)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C52),ISBLANK(C53)),\"\",IF(OR(ISBLANK(C53),ISBLANK(C52)),AVERAGE(F52:F53),IF(OR(ISTEXT(F52),ISTEXT(F53)),\"Invalid\",AVERAGE(F52:F53))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C54),ISBLANK(C55)),\"\",IF(AVERAGE($C54:$C55)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C54:$C55)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C54),ISBLANK(C55)),\"\",STDEV(C54,C55))","v":""},{"$f":"IF(ISTEXT(D55),\"\",D55/((C54+C55)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C54),ISBLANK(C55)),\"\",IF(OR(ISBLANK(C55),ISBLANK(C54)),AVERAGE(F54:F55),IF(OR(ISTEXT(F54),ISTEXT(F55)),\"Invalid\",AVERAGE(F54:F55))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C56),\"\",IF(C56<=$C$31,\"Invalid\",IF(C56>=$C$28,\"Invalid\",$C$30*(($C$28-C56)/(C56-$C$31))^(1/$C$29))))","v":""},null,{"$f":"IF(AND(ISBLANK(C56),ISBLANK(C57)),\"\",IF(AVERAGE($C56:$C57)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C56:$C57)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C56),ISBLANK(C57)),\"\",STDEV(C56,C57))","v":""},{"$f":"IF(ISTEXT(D57),\"\",D57/((C56+C57)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C56),ISBLANK(C57)),\"\",IF(OR(ISBLANK(C57),ISBLANK(C56)),AVERAGE(F56:F57),IF(OR(ISTEXT(F56),ISTEXT(F57)),\"Invalid\",AVERAGE(F56:F57))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C58),ISBLANK(C59)),\"\",IF(AVERAGE($C58:$C59)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C58:$C59)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C58),ISBLANK(C59)),\"\",STDEV(C58,C59))","v":""},{"$f":"IF(ISTEXT(D59),\"\",D59/((C58+C59)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C58),ISBLANK(C59)),\"\",IF(OR(ISBLANK(C59),ISBLANK(C58)),AVERAGE(F58:F59),IF(OR(ISTEXT(F58),ISTEXT(F59)),\"Invalid\",AVERAGE(F58:F59))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C60),ISBLANK(C61)),\"\",IF(AVERAGE($C60:$C61)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C60:$C61)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C60),ISBLANK(C61)),\"\",STDEV(C60,C61))","v":""},{"$f":"IF(ISTEXT(D61),\"\",D61/((C60+C61)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C60),ISBLANK(C61)),\"\",IF(OR(ISBLANK(C61),ISBLANK(C60)),AVERAGE(F60:F61),IF(OR(ISTEXT(F60),ISTEXT(F61)),\"Invalid\",AVERAGE(F60:F61))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C62),ISBLANK(C63)),\"\",IF(AVERAGE($C62:$C63)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C62:$C63)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C62),ISBLANK(C63)),\"\",STDEV(C62,C63))","v":""},{"$f":"IF(ISTEXT(D63),\"\",D63/((C62+C63)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C62),ISBLANK(C63)),\"\",IF(OR(ISBLANK(C63),ISBLANK(C62)),AVERAGE(F62:F63),IF(OR(ISTEXT(F62),ISTEXT(F63)),\"Invalid\",AVERAGE(F62:F63))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C64),ISBLANK(C65)),\"\",IF(AVERAGE($C64:$C65)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C64:$C65)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C64),ISBLANK(C65)),\"\",STDEV(C64,C65))","v":""},{"$f":"IF(ISTEXT(D65),\"\",D65/((C64+C65)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C64),ISBLANK(C65)),\"\",IF(OR(ISBLANK(C65),ISBLANK(C64)),AVERAGE(F64:F65),IF(OR(ISTEXT(F64),ISTEXT(F65)),\"Invalid\",AVERAGE(F64:F65))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C66),ISBLANK(C67)),\"\",IF(AVERAGE($C66:$C67)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C66:$C67)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C66),ISBLANK(C67)),\"\",STDEV(C66,C67))","v":""},{"$f":"IF(ISTEXT(D67),\"\",D67/((C66+C67)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C66),ISBLANK(C67)),\"\",IF(OR(ISBLANK(C67),ISBLANK(C66)),AVERAGE(F66:F67),IF(OR(ISTEXT(F66),ISTEXT(F67)),\"Invalid\",AVERAGE(F66:F67))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C68),ISBLANK(C69)),\"\",IF(AVERAGE($C68:$C69)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C68:$C69)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C68),ISBLANK(C69)),\"\",STDEV(C68,C69))","v":""},{"$f":"IF(ISTEXT(D69),\"\",D69/((C68+C69)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C68),ISBLANK(C69)),\"\",IF(OR(ISBLANK(C69),ISBLANK(C68)),AVERAGE(F68:F69),IF(OR(ISTEXT(F68),ISTEXT(F69)),\"Invalid\",AVERAGE(F68:F69))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C70),ISBLANK(C71)),\"\",IF(AVERAGE($C70:$C71)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C70:$C71)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
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[null,null,null,{"$f":"IF(OR(ISBLANK(C118),ISBLANK(C119)),\"\",STDEV(C118,C119))","v":""},{"$f":"IF(ISTEXT(D119),\"\",D119/((C118+C119)/2)*100)","v":""},{"$f":"IF(ISBLANK(C119),\"\",IF(C119<=$C$31,\"invalid\",IF(C119>=$C$28,\"invalid\",$C$30*(($C$28-C119)/(C119-$C$31))^(1/$C$29))))","v":""},{"$f":"IF(AND(ISBLANK(C118),ISBLANK(C119)),\"\",IF(OR(ISBLANK(C119),ISBLANK(C118)),AVERAGE(F118:F119),IF(OR(ISTEXT(F118),ISTEXT(F119)),\"Invalid\",AVERAGE(F118:F119))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C120),\"\",IF(C120<=$C$31,\"Invalid\",IF(C120>=$C$28,\"Invalid\",$C$30*(($C$28-C120)/(C120-$C$31))^(1/$C$29))))","v":""},null,{"$f":"IF(AND(ISBLANK(C120),ISBLANK(C121)),\"\",IF(AVERAGE($C120:$C121)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C120:$C121)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C120),ISBLANK(C121)),\"\",STDEV(C120,C121))","v":""},{"$f":"IF(ISTEXT(D121),\"\",D121/((C120+C121)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C120),ISBLANK(C121)),\"\",IF(OR(ISBLANK(C121),ISBLANK(C120)),AVERAGE(F120:F121),IF(OR(ISTEXT(F120),ISTEXT(F121)),\"Invalid\",AVERAGE(F120:F121))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C122),ISBLANK(C123)),\"\",IF(AVERAGE($C122:$C123)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C122:$C123)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C122),ISBLANK(C123)),\"\",STDEV(C122,C123))","v":""},{"$f":"IF(ISTEXT(D123),\"\",D123/((C122+C123)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C122),ISBLANK(C123)),\"\",IF(OR(ISBLANK(C123),ISBLANK(C122)),AVERAGE(F122:F123),IF(OR(ISTEXT(F122),ISTEXT(F123)),\"Invalid\",AVERAGE(F122:F123))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C124),\"\",IF(C124<=$C$31,\"Invalid\",IF(C124>=$C$28,\"Invalid\",$C$30*(($C$28-C124)/(C124-$C$31))^(1/$C$29))))","v":""},null,{"$f":"IF(AND(ISBLANK(C124),ISBLANK(C125)),\"\",IF(AVERAGE($C124:$C125)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C124:$C125)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C124),ISBLANK(C125)),\"\",STDEV(C124,C125))","v":""},{"$f":"IF(ISTEXT(D125),\"\",D125/((C124+C125)/2)*100)","v":""},{"$f":"IF(ISBLANK(C125),\"\",IF(C125<=$C$31,\"Invalid\",IF(C125>=$C$28,\"Invalid\",$C$30*(($C$28-C125)/(C125-$C$31))^(1/$C$29))))","v":""},{"$f":"IF(AND(ISBLANK(C124),ISBLANK(C125)),\"\",IF(OR(ISBLANK(C125),ISBLANK(C124)),AVERAGE(F124:F125),IF(OR(ISTEXT(F124),ISTEXT(F125)),\"Invalid\",AVERAGE(F124:F125))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C126),ISBLANK(C127)),\"\",IF(AVERAGE($C126:$C127)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C126:$C127)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C126),ISBLANK(C127)),\"\",STDEV(C126,C127))","v":""},{"$f":"IF(ISTEXT(D127),\"\",D127/((C126+C127)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C126),ISBLANK(C127)),\"\",IF(OR(ISBLANK(C127),ISBLANK(C126)),AVERAGE(F126:F127),IF(OR(ISTEXT(F126),ISTEXT(F127)),\"Invalid\",AVERAGE(F126:F127))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C128),\"\",IF(C128<=$C$31,\"Invalid\",IF(C128>=$C$28,\"Invalid\",$C$30*(($C$28-C128)/(C128-$C$31))^(1/$C$29))))","v":""},null,{"$f":"IF(AND(ISBLANK(C128),ISBLANK(C129)),\"\",IF(AVERAGE($C128:$C129)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C128:$C129)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C128),ISBLANK(C129)),\"\",STDEV(C128,C129))","v":""},{"$f":"IF(ISTEXT(D129),\"\",D129/((C128+C129)/2)*100)","v":""},{"$f":"IF(ISBLANK(C129),\"\",IF(C129<=$C$31,\"Invalid\",IF(C129>=$C$28,\"Invalid\",$C$30*(($C$28-C129)/(C129-$C$31))^(1/$C$29))))","v":""},{"$f":"IF(AND(ISBLANK(C128),ISBLANK(C129)),\"\",IF(OR(ISBLANK(C129),ISBLANK(C128)),AVERAGE(F128:F129),IF(OR(ISTEXT(F128),ISTEXT(F129)),\"Invalid\",AVERAGE(F128:F129))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C130),ISBLANK(C131)),\"\",IF(AVERAGE($C130:$C131)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C130:$C131)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C130),ISBLANK(C131)),\"\",STDEV(C130,C131))","v":""},{"$f":"IF(ISTEXT(D131),\"\",D131/((C130+C131)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C130),ISBLANK(C131)),\"\",IF(OR(ISBLANK(C131),ISBLANK(C130)),AVERAGE(F130:F131),IF(OR(ISTEXT(F130),ISTEXT(F131)),\"Invalid\",AVERAGE(F130:F131))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C132),ISBLANK(C133)),\"\",IF(AVERAGE($C132:$C133)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C132:$C133)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C132),ISBLANK(C133)),\"\",STDEV(C132,C133))","v":""},{"$f":"IF(ISTEXT(D133),\"\",D133/((C132+C133)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C132),ISBLANK(C133)),\"\",IF(OR(ISBLANK(C133),ISBLANK(C132)),AVERAGE(F132:F133),IF(OR(ISTEXT(F132),ISTEXT(F133)),\"Invalid\",AVERAGE(F132:F133))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C134),ISBLANK(C135)),\"\",IF(AVERAGE($C134:$C135)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C134:$C135)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C134),ISBLANK(C135)),\"\",STDEV(C134,C135))","v":""},{"$f":"IF(ISTEXT(D135),\"\",D135/((C134+C135)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C134),ISBLANK(C135)),\"\",IF(OR(ISBLANK(C135),ISBLANK(C134)),AVERAGE(F134:F135),IF(OR(ISTEXT(F134),ISTEXT(F135)),\"Invalid\",AVERAGE(F134:F135))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C136),ISBLANK(C137)),\"\",IF(AVERAGE($C136:$C137)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C136:$C137)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C136),ISBLANK(C137)),\"\",STDEV(C136,C137))","v":""},{"$f":"IF(ISTEXT(D137),\"\",D137/((C136+C137)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C136),ISBLANK(C137)),\"\",IF(OR(ISBLANK(C137),ISBLANK(C136)),AVERAGE(F136:F137),IF(OR(ISTEXT(F136),ISTEXT(F137)),\"Invalid\",AVERAGE(F136:F137))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C138),ISBLANK(C139)),\"\",IF(AVERAGE($C138:$C139)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C138:$C139)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C138),ISBLANK(C139)),\"\",STDEV(C138,C139))","v":""},{"$f":"IF(ISTEXT(D139),\"\",D139/((C138+C139)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C138),ISBLANK(C139)),\"\",IF(OR(ISBLANK(C139),ISBLANK(C138)),AVERAGE(F138:F139),IF(OR(ISTEXT(F138),ISTEXT(F139)),\"Invalid\",AVERAGE(F138:F139))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C140),ISBLANK(C141)),\"\",IF(AVERAGE($C140:$C141)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C140:$C141)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C140),ISBLANK(C141)),\"\",STDEV(C140,C141))","v":""},{"$f":"IF(ISTEXT(D141),\"\",D141/((C140+C141)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C140),ISBLANK(C141)),\"\",IF(OR(ISBLANK(C141),ISBLANK(C140)),AVERAGE(F140:F141),IF(OR(ISTEXT(F140),ISTEXT(F141)),\"Invalid\",AVERAGE(F140:F141))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C142),ISBLANK(C143)),\"\",IF(AVERAGE($C142:$C143)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C142:$C143)<$D$24,\"GREATER than Standard 3, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C142),ISBLANK(C143)),\"\",STDEV(C142,C143))","v":""},{"$f":"IF(ISTEXT(D143),\"\",D143/((C142+C143)/2)*100)","v":""},{"$f":"IF(ISBLANK(C143),\"\",IF(C143<=$C$31,\"invalid\",IF(C143>=$C$28,\"invalid\",$C$30*(($C$28-C143)/(C143-$C$31))^(1/$C$29))))","v":""},{"$f":"IF(AND(ISBLANK(C142),ISBLANK(C143)),\"\",IF(OR(ISBLANK(C143),ISBLANK(C142)),AVERAGE(F142:F143),IF(OR(ISTEXT(F142),ISTEXT(F143)),\"Invalid\",AVERAGE(F142:F143))))","v":""}]

{"sheet":"5 Standards","index":2,"n_rows":144,"n_cols":19,"rows_kept":144,"cut":false,"hidden":false,"merged":[[66,7,67,9],[68,7,69,9],[70,7,71,9],[54,7,55,9],[56,7,57,9],[58,7,59,9],[60,7,61,9],[62,7,63,9],[64,7,65,9],[52,7,53,9],[14,15,14,24],[16,15,16,24],[35,7,35,9],[36,7,37,9],[38,7,39,9],[40,7,41,9],[18,1,18,7],[34,1,34,9],[42,7,43,9],[44,7,45,9],[46,7,47,9],[48,7,49,9],[50,7,51,9],[13,15,13,24],[1,1,1,5],[1,15,1,24],[3,15,3,24],[5,15,5,24],[6,15,6,24],[7,15,7,24],[8,15,8,24],[9,15,9,24],[11,15,11,24],[12,15,12,24],[3,1,3,5],[72,7,73,9],[74,7,75,9],[76,7,77,9],[78,7,79,9],[80,7,81,9],[82,7,83,9],[84,7,85,9],[86,7,87,9],[88,7,89,9],[90,7,91,9],[92,7,93,9],[94,7,95,9],[96,7,97,9],[98,7,99,9],[100,7,101,9],[102,7,103,9],[104,7,105,9],[106,7,107,9],[108,7,109,9],[110,7,111,9],[112,7,113,9],[114,7,115,9],[116,7,117,9],[118,7,119,9],[120,7,121,9],[122,7,123,9],[124,7,125,9],[126,7,127,9],[128,7,129,9],[130,7,131,9],[142,7,143,9],[132,7,133,9],[134,7,135,9],[136,7,137,9],[138,7,139,9],[140,7,141,9]]}
[]
[null,"** USER MUST ONLY INPUT VALUES IN GREY-COLORED CELLS **",null,null,null,null,"Kit Lot #",null,null,null,null,null,null,null,null,"Before you start:"]
[]
[null,"Enter the absorbance values of each standard into the grey boxes",null,null,null,null,null,null,null,null,null,null,null,null,null,"Check that \"solver\" is installed"]
[null,"Std 0","Std 1","Std 2","Std 3","Std 4"]
[null,1.85,1.55,1.25,0.95,0.75,null,null,null,null,null,null,5.8999999999999997E-2,null,null,"Input OD values for standards (Fig 4 in protocol)"]
[null,1.83,1.53,1.23,0.93,0.73,null,null,null,null,null,null,6.0999999999999999E-2,null,null,"Select solver  (Target Cell: $e$32 - Cell Changes: $c$35:$c$38 - Optimize: Minimize - GRG Nonlinear )"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"\"Solve\""]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Check graph and parametres (A>0.7; D<0.1; "]
[]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Calculate MC TADDA-concentration in samples in wells: "]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Input average OD of unknown samples in cell c48 (sample 1) , c49 (sample 2)…."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Calculated ELISA MCTADDA concentration are given automatically in cell d48 (sample 1), c49 (sample 2),…."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Sample concentrations should only be used from datapoints that are within the valid working range of the standard curve"]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"If OD is outsite the Ic20 to Ic80 range, dilution sholud be adjusted and the samples assayed again"]
[]
[null,"Enter the standard concentrations of each standard into the grey boxes"]
[null,"Standards (ng/ml)","OD (averaged)","Predicted values","Residual Squares","B/Bo","Std Dev","%CV"]
[null,0,{"$f":"(B6+B7)/2","v":1.84},{"$f":"($C$29-$C$32)/(1+(B21/$C$31)^$C$30)+$C$32","v":1.8406360655818004},{"$f":"(C21-D21)^2","v":4.0457942435094599E-7},null,{"$f":"STDEV(B6,B7)","v":1.4142135623730963E-2},{"$f":"(G21/C21)*100","v":0.76859432737668276}]
[null,0.15,{"$f":"(C6+C7)/2","v":1.54},{"$f":"($C$29-$C$32)/(1+(B22/$C$31)^$C$30)+$C$32","v":1.5366981700967135},1.0902080710237169E-5,{"$f":"C22/C$21","v":0.83695652173913038},{"$f":"STDEV(C6,C7)","v":1.4142135623730963E-2},0.91832049504746505,null,null,null,null,null,null,null,null,"OD","MCTADDA-conc","X=C x ((A-Y)/(Y-D))^(1/B)"]
[null,0.4,{"$f":"(D6+D7)/2","v":1.24},{"$f":"($C$29-$C$32)/(1+(B23/$C$31)^$C$30)+$C$32","v":1.2460146394820644},3.6175888099207637E-5,{"$f":"C23/C$21","v":0.67391304347826086},{"$f":"STDEV(D6,D7)","v":1.4142135623730963E-2},1.1404948083654003,null,null,null,null,null,null,null,"Ic20",{"$f":"$C$32+($C$29-$C$32)*20/100","v":0.72666598968887874},{"$f":"C31*((C29-Q23)/(Q23-C32))^(1/C30)","v":2.1433652820942983}]
[null,1,{"$f":"(E6+E7)/2","v":0.94},{"$f":"($C$29-$C$32)/(1+(B24/$C$31)^$C$30)+$C$32","v":0.93422270428784371},3.3377145745698858E-5,{"$f":"C24/C$21","v":0.51086956521739124},{"$f":"STDEV(E6,E7)","v":1.4142135623730885E-2},1.5044825131628601,null,null,null,null,null,null,null,"Ic80",{"$f":"$C$32+($C$29-$C$32)*80/100","v":1.5621435466085698},{"$f":"C31*((C29-Q24)/(Q24-C32))^(1/C30)","v":0.13431692258505473}]
[null,2,{"$f":"(F6+F7)/2","v":0.74},{"$f":"($C$29-$C$32)/(1+(B25/$C$31)^$C$30)+$C$32","v":0.74242614727460787},5.8861905980872517E-6,{"$f":"C25/C$21","v":0.40217391304347822},{"$f":"STDEV(F6,F7)","v":1.4142135623730963E-2},1.9110994086122923]
[null,"Sum Of Squares",null,null,{"$f":"SUM(E21:E25)","v":8.6745884577581862E-5}]
[]
[null,"Parameter (Y=(A-D)/(1+(X/C)^B)+D)",null,null,"R-Squared"]
[null,"A",1.8406360655818004,"(Max.)",{"$f":"(CORREL(C21:C25,D21:D25))^2","v":0.9998899163979047}]
[null,"B",1.0009597313993301,"(Slope)"]
[null,"C",0.53655403145121705,"(IC50)"]
[null,"D",0.44817347071564828,"(Min.)"]
[]
[]
[null,"Enter descriptions and absorbance values of each sample and control into the grey boxes"]
[null,"Sample/Control","Absorbances","Std Dev","%CV","Sample Concentration (ng/ml)","Sample Concentration Avg (ng/ml)","Comments"]
[null,"Control",1.03,null,null,{"$f":"IF(ISBLANK(C37),\"\",IF(C37<=$C$32,\"Invalid\",IF(C37>=$C$29,\"Invalid\",$C$31*(($C$29-C37)/(C37-$C$32))^(1/$C$30))))","v":0.74732199511182373},null,{"$f":"IF(AND(ISBLANK(C37),ISBLANK(C38)),\"\",IF(AVERAGE($C37:$C38)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C37:$C38)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,"Control",1.05,{"$f":"IF(OR(ISBLANK(C37),ISBLANK(C38)),\"\",STDEV(C37,C38))","v":1.4142135623730963E-2},{"$f":"IF(ISTEXT(D38),\"\",D38/((C37+C38)/2)*100)","v":1.3598207330510541},{"$f":"IF(ISBLANK(C38),\"\",IF(C38<=$C$32,\"Invalid\",IF(C38>=$C$29,\"Invalid\",$C$31*(($C$29-C38)/(C38-$C$32))^(1/$C$30))))","v":0.70470139504982643},{"$f":"IF(AND(ISBLANK(C37),ISBLANK(C38)),\"\",IF(OR(ISBLANK(C38),ISBLANK(C37)),AVERAGE(F37:F38),IF(OR(ISTEXT(F37),ISTEXT(F38)),\"Invalid\",AVERAGE(F37:F38))))","v":0.72601169508082508}]
[null,"Sample 1",1.23,null,null,0.41916828690019042,null,{"$f":"IF(AND(ISBLANK(C39),ISBLANK(C40)),\"\",IF(AVERAGE($C39:$C40)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C39:$C40)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,"Sample 1",1.25,{"$f":"IF(OR(ISBLANK(C39),ISBLANK(C40)),\"\",STDEV(C39,C40))","v":1.4142135623730963E-2},{"$f":"IF(ISTEXT(D40),\"\",D40/((C39+C40)/2)*100)","v":1.1404948083654003},0.39534868240609067,{"$f":"IF(AND(ISBLANK(C39),ISBLANK(C40)),\"\",IF(OR(ISBLANK(C40),ISBLANK(C39)),AVERAGE(F39:F40),IF(OR(ISTEXT(F39),ISTEXT(F40)),\"Invalid\",AVERAGE(F39:F40))))","v":0.40725848465314052}]
[null,"Sample 2",0.85,null,null,1.3216402502729721,null,{"$f":"IF(AND(ISBLANK(C41),ISBLANK(C42)),\"\",IF(AVERAGE($C41:$C42)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C41:$C42)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,"Sample 2",0.83,{"$f":"IF(OR(ISBLANK(C41),ISBLANK(C42)),\"\",STDEV(C41,C42))","v":1.4142135623730963E-2},{"$f":"IF(ISTEXT(D42),\"\",D42/((C41+C42)/2)*100)","v":1.6835875742536861},1.4188511544507727,{"$f":"IF(AND(ISBLANK(C41),ISBLANK(C42)),\"\",IF(OR(ISBLANK(C42),ISBLANK(C41)),AVERAGE(F41:F42),IF(OR(ISTEXT(F41),ISTEXT(F42)),\"Invalid\",AVERAGE(F41:F42))))","v":1.3702457023618724}]
[null,"Sample 3",1.63,null,null,9.5787895445484356E-2,null,{"$f":"IF(AND(ISBLANK(C43),ISBLANK(C44)),\"\",IF(AVERAGE($C43:$C44)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C43:$C44)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":"LESS than Standard 1, outside of quantifiable range"}]
[null,"Sample 3",1.65,{"$f":"IF(OR(ISBLANK(C43),ISBLANK(C44)),\"\",STDEV(C43,C44))","v":1.4142135623730963E-2},{"$f":"IF(ISTEXT(D44),\"\",D44/((C43+C44)/2)*100)","v":0.86232534291042462},8.5259629842804108E-2,{"$f":"IF(AND(ISBLANK(C43),ISBLANK(C44)),\"\",IF(OR(ISBLANK(C44),ISBLANK(C43)),AVERAGE(F43:F44),IF(OR(ISTEXT(F43),ISTEXT(F44)),\"Invalid\",AVERAGE(F43:F44))))","v":9.0523762644144232E-2}]
[null,"Sample 4",0.52,null,null,9.8378301251336584,null,{"$f":"IF(AND(ISBLANK(C45),ISBLANK(C46)),\"\",IF(AVERAGE($C45:$C46)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C45:$C46)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":"GREATER than Standard 4, outside of quantifiable range"}]
[null,"Sample 4",0.51,{"$f":"IF(OR(ISBLANK(C45),ISBLANK(C46)),\"\",STDEV(C45,C46))","v":7.0710678118654814E-3},{"$f":"IF(ISTEXT(D46),\"\",D46/((C45+C46)/2)*100)","v":1.3730228760903846},11.513832329463826,{"$f":"IF(AND(ISBLANK(C45),ISBLANK(C46)),\"\",IF(OR(ISBLANK(C46),ISBLANK(C45)),AVERAGE(F45:F46),IF(OR(ISTEXT(F45),ISTEXT(F46)),\"Invalid\",AVERAGE(F45:F46))))","v":10.675831227298742}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C47),ISBLANK(C48)),\"\",IF(AVERAGE($C47:$C48)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C47:$C48)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C47),ISBLANK(C48)),\"\",STDEV(C47,C48))","v":""},{"$f":"IF(ISTEXT(D48),\"\",D48/((C47+C48)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C47),ISBLANK(C48)),\"\",IF(OR(ISBLANK(C48),ISBLANK(C47)),AVERAGE(F47:F48),IF(OR(ISTEXT(F47),ISTEXT(F48)),\"Invalid\",AVERAGE(F47:F48))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C49),ISBLANK(C50)),\"\",IF(AVERAGE($C49:$C50)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C49:$C50)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C49),ISBLANK(C50)),\"\",STDEV(C49,C50))","v":""},{"$f":"IF(ISTEXT(D50),\"\",D50/((C49+C50)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C49),ISBLANK(C50)),\"\",IF(OR(ISBLANK(C50),ISBLANK(C49)),AVERAGE(F49:F50),IF(OR(ISTEXT(F49),ISTEXT(F50)),\"Invalid\",AVERAGE(F49:F50))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C51),ISBLANK(C52)),\"\",IF(AVERAGE($C51:$C52)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C51:$C52)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C51),ISBLANK(C52)),\"\",STDEV(C51,C52))","v":""},{"$f":"IF(ISTEXT(D52),\"\",D52/((C51+C52)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C51),ISBLANK(C52)),\"\",IF(OR(ISBLANK(C52),ISBLANK(C51)),AVERAGE(F51:F52),IF(OR(ISTEXT(F51),ISTEXT(F52)),\"Invalid\",AVERAGE(F51:F52))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C53),\"\",IF(C53<=$C$32,\"Invalid\",IF(C53>=$C$29,\"Invalid\",$C$31*(($C$29-C53)/(C53-$C$32))^(1/$C$30))))","v":""},null,{"$f":"IF(AND(ISBLANK(C53),ISBLANK(C54)),\"\",IF(AVERAGE($C53:$C54)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C53:$C54)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C53),ISBLANK(C54)),\"\",STDEV(C53,C54))","v":""},{"$f":"IF(ISTEXT(D54),\"\",D54/((C53+C54)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C53),ISBLANK(C54)),\"\",IF(OR(ISBLANK(C54),ISBLANK(C53)),AVERAGE(F53:F54),IF(OR(ISTEXT(F53),ISTEXT(F54)),\"Invalid\",AVERAGE(F53:F54))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C55),ISBLANK(C56)),\"\",IF(AVERAGE($C55:$C56)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C55:$C56)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C55),ISBLANK(C56)),\"\",STDEV(C55,C56))","v":""},{"$f":"IF(ISTEXT(D56),\"\",D56/((C55+C56)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C55),ISBLANK(C56)),\"\",IF(OR(ISBLANK(C56),ISBLANK(C55)),AVERAGE(F55:F56),IF(OR(ISTEXT(F55),ISTEXT(F56)),\"Invalid\",AVERAGE(F55:F56))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C57),\"\",IF(C57<=$C$32,\"Invalid\",IF(C57>=$C$29,\"Invalid\",$C$31*(($C$29-C57)/(C57-$C$32))^(1/$C$30))))","v":""},null,{"$f":"IF(AND(ISBLANK(C57),ISBLANK(C58)),\"\",IF(AVERAGE($C57:$C58)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C57:$C58)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C57),ISBLANK(C58)),\"\",STDEV(C57,C58))","v":""},{"$f":"IF(ISTEXT(D58),\"\",D58/((C57+C58)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C57),ISBLANK(C58)),\"\",IF(OR(ISBLANK(C58),ISBLANK(C57)),AVERAGE(F57:F58),IF(OR(ISTEXT(F57),ISTEXT(F58)),\"Invalid\",AVERAGE(F57:F58))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C59),ISBLANK(C60)),\"\",IF(AVERAGE($C59:$C60)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C59:$C60)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C59),ISBLANK(C60)),\"\",STDEV(C59,C60))","v":""},{"$f":"IF(ISTEXT(D60),\"\",D60/((C59+C60)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C59),ISBLANK(C60)),\"\",IF(OR(ISBLANK(C60),ISBLANK(C59)),AVERAGE(F59:F60),IF(OR(ISTEXT(F59),ISTEXT(F60)),\"Invalid\",AVERAGE(F59:F60))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C61),ISBLANK(C62)),\"\",IF(AVERAGE($C61:$C62)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C61:$C62)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C61),ISBLANK(C62)),\"\",STDEV(C61,C62))","v":""},{"$f":"IF(ISTEXT(D62),\"\",D62/((C61+C62)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C61),ISBLANK(C62)),\"\",IF(OR(ISBLANK(C62),ISBLANK(C61)),AVERAGE(F61:F62),IF(OR(ISTEXT(F61),ISTEXT(F62)),\"Invalid\",AVERAGE(F61:F62))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C63),ISBLANK(C64)),\"\",IF(AVERAGE($C63:$C64)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C63:$C64)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C63),ISBLANK(C64)),\"\",STDEV(C63,C64))","v":""},{"$f":"IF(ISTEXT(D64),\"\",D64/((C63+C64)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C63),ISBLANK(C64)),\"\",IF(OR(ISBLANK(C64),ISBLANK(C63)),AVERAGE(F63:F64),IF(OR(ISTEXT(F63),ISTEXT(F64)),\"Invalid\",AVERAGE(F63:F64))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C65),ISBLANK(C66)),\"\",IF(AVERAGE($C65:$C66)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C65:$C66)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C65),ISBLANK(C66)),\"\",STDEV(C65,C66))","v":""},{"$f":"IF(ISTEXT(D66),\"\",D66/((C65+C66)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C65),ISBLANK(C66)),\"\",IF(OR(ISBLANK(C66),ISBLANK(C65)),AVERAGE(F65:F66),IF(OR(ISTEXT(F65),ISTEXT(F66)),\"Invalid\",AVERAGE(F65:F66))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C67),ISBLANK(C68)),\"\",IF(AVERAGE($C67:$C68)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C67:$C68)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C67),ISBLANK(C68)),\"\",STDEV(C67,C68))","v":""},{"$f":"IF(ISTEXT(D68),\"\",D68/((C67+C68)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C67),ISBLANK(C68)),\"\",IF(OR(ISBLANK(C68),ISBLANK(C67)),AVERAGE(F67:F68),IF(OR(ISTEXT(F67),ISTEXT(F68)),\"Invalid\",AVERAGE(F67:F68))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C69),ISBLANK(C70)),\"\",IF(AVERAGE($C69:$C70)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C69:$C70)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C69),ISBLANK(C70)),\"\",STDEV(C69,C70))","v":""},{"$f":"IF(ISTEXT(D70),\"\",D70/((C69+C70)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C69),ISBLANK(C70)),\"\",IF(OR(ISBLANK(C70),ISBLANK(C69)),AVERAGE(F69:F70),IF(OR(ISTEXT(F69),ISTEXT(F70)),\"Invalid\",AVERAGE(F69:F70))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C71),ISBLANK(C72)),\"\",IF(AVERAGE($C71:$C72)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C71:$C72)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C71),ISBLANK(C72)),\"\",STDEV(C71,C72))","v":""},{"$f":"IF(ISTEXT(D72),\"\",D72/((C71+C72)/2)*100)","v":""},{"$f":"IF(ISBLANK(C72),\"\",IF(C72<=$C$32,\"invalid\",IF(C72>=$C$29,\"invalid\",$C$31*(($C$29-C72)/(C72-$C$32))^(1/$C$30))))","v":""},{"$f":"IF(AND(ISBLANK(C71),ISBLANK(C72)),\"\",IF(OR(ISBLANK(C72),ISBLANK(C71)),AVERAGE(F71:F72),IF(OR(ISTEXT(F71),ISTEXT(F72)),\"Invalid\",AVERAGE(F71:F72))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C73),\"\",IF(C73<=$C$32,\"Invalid\",IF(C73>=$C$29,\"Invalid\",$C$31*(($C$29-C73)/(C73-$C$32))^(1/$C$30))))","v":""},null,{"$f":"IF(AND(ISBLANK(C73),ISBLANK(C74)),\"\",IF(AVERAGE($C73:$C74)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C73:$C74)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C73),ISBLANK(C74)),\"\",STDEV(C73,C74))","v":""},{"$f":"IF(ISTEXT(D74),\"\",D74/((C73+C74)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C73),ISBLANK(C74)),\"\",IF(OR(ISBLANK(C74),ISBLANK(C73)),AVERAGE(F73:F74),IF(OR(ISTEXT(F73),ISTEXT(F74)),\"Invalid\",AVERAGE(F73:F74))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C75),ISBLANK(C76)),\"\",IF(AVERAGE($C75:$C76)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C75:$C76)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C75),ISBLANK(C76)),\"\",STDEV(C75,C76))","v":""},{"$f":"IF(ISTEXT(D76),\"\",D76/((C75+C76)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C75),ISBLANK(C76)),\"\",IF(OR(ISBLANK(C76),ISBLANK(C75)),AVERAGE(F75:F76),IF(OR(ISTEXT(F75),ISTEXT(F76)),\"Invalid\",AVERAGE(F75:F76))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C77),\"\",IF(C77<=$C$32,\"Invalid\",IF(C77>=$C$29,\"Invalid\",$C$31*(($C$29-C77)/(C77-$C$32))^(1/$C$30))))","v":""},null,{"$f":"IF(AND(ISBLANK(C77),ISBLANK(C78)),\"\",IF(AVERAGE($C77:$C78)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C77:$C78)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C77),ISBLANK(C78)),\"\",STDEV(C77,C78))","v":""},{"$f":"IF(ISTEXT(D78),\"\",D78/((C77+C78)/2)*100)","v":""},{"$f":"IF(ISBLANK(C78),\"\",IF(C78<=$C$32,\"Invalid\",IF(C78>=$C$29,\"Invalid\",$C$31*(($C$29-C78)/(C78-$C$32))^(1/$C$30))))","v":""},{"$f":"IF(AND(ISBLANK(C77),ISBLANK(C78)),\"\",IF(OR(ISBLANK(C78),ISBLANK(C77)),AVERAGE(F77:F78),IF(OR(ISTEXT(F77),ISTEXT(F78)),\"Invalid\",AVERAGE(F77:F78))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C79),ISBLANK(C80)),\"\",IF(AVERAGE($C79:$C80)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C79:$C80)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C79),ISBLANK(C80)),\"\",STDEV(C79,C80))","v":""},{"$f":"IF(ISTEXT(D80),\"\",D80/((C79+C80)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C79),ISBLANK(C80)),\"\",IF(OR(ISBLANK(C80),ISBLANK(C79)),AVERAGE(F79:F80),IF(OR(ISTEXT(F79),ISTEXT(F80)),\"Invalid\",AVERAGE(F79:F80))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C81),\"\",IF(C81<=$C$32,\"Invalid\",IF(C81>=$C$29,\"Invalid\",$C$31*(($C$29-C81)/(C81-$C$32))^(1/$C$30))))","v":""},null,{"$f":"IF(AND(ISBLANK(C81),ISBLANK(C82)),\"\",IF(AVERAGE($C81:$C82)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C81:$C82)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C81),ISBLANK(C82)),\"\",STDEV(C81,C82))","v":""},{"$f":"IF(ISTEXT(D82),\"\",D82/((C81+C82)/2)*100)","v":""},{"$f":"IF(ISBLANK(C82),\"\",IF(C82<=$C$32,\"Invalid\",IF(C82>=$C$29,\"Invalid\",$C$31*(($C$29-C82)/(C82-$C$32))^(1/$C$30))))","v":""},{"$f":"IF(AND(ISBLANK(C81),ISBLANK(C82)),\"\",IF(OR(ISBLANK(C82),ISBLANK(C81)),AVERAGE(F81:F82),IF(OR(ISTEXT(F81),ISTEXT(F82)),\"Invalid\",AVERAGE(F81:F82))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C83),ISBLANK(C84)),\"\",IF(AVERAGE($C83:$C84)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C83:$C84)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C83),ISBLANK(C84)),\"\",STDEV(C83,C84))","v":""},{"$f":"IF(ISTEXT(D84),\"\",D84/((C83+C84)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C83),ISBLANK(C84)),\"\",IF(OR(ISBLANK(C84),ISBLANK(C83)),AVERAGE(F83:F84),IF(OR(ISTEXT(F83),ISTEXT(F84)),\"Invalid\",AVERAGE(F83:F84))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C85),ISBLANK(C86)),\"\",IF(AVERAGE($C85:$C86)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C85:$C86)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C85),ISBLANK(C86)),\"\",STDEV(C85,C86))","v":""},{"$f":"IF(ISTEXT(D86),\"\",D86/((C85+C86)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C85),ISBLANK(C86)),\"\",IF(OR(ISBLANK(C86),ISBLANK(C85)),AVERAGE(F85:F86),IF(OR(ISTEXT(F85),ISTEXT(F86)),\"Invalid\",AVERAGE(F85:F86))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C87),ISBLANK(C88)),\"\",IF(AVERAGE($C87:$C88)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C87:$C88)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C87),ISBLANK(C88)),\"\",STDEV(C87,C88))","v":""},{"$f":"IF(ISTEXT(D88),\"\",D88/((C87+C88)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C87),ISBLANK(C88)),\"\",IF(OR(ISBLANK(C88),ISBLANK(C87)),AVERAGE(F87:F88),IF(OR(ISTEXT(F87),ISTEXT(F88)),\"Invalid\",AVERAGE(F87:F88))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C89),ISBLANK(C90)),\"\",IF(AVERAGE($C89:$C90)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C89:$C90)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C89),ISBLANK(C90)),\"\",STDEV(C89,C90))","v":""},{"$f":"IF(ISTEXT(D90),\"\",D90/((C89+C90)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C89),ISBLANK(C90)),\"\",IF(OR(ISBLANK(C90),ISBLANK(C89)),AVERAGE(F89:F90),IF(OR(ISTEXT(F89),ISTEXT(F90)),\"Invalid\",AVERAGE(F89:F90))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C91),ISBLANK(C92)),\"\",IF(AVERAGE($C91:$C92)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C91:$C92)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C91),ISBLANK(C92)),\"\",STDEV(C91,C92))","v":""},{"$f":"IF(ISTEXT(D92),\"\",D92/((C91+C92)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C91),ISBLANK(C92)),\"\",IF(OR(ISBLANK(C92),ISBLANK(C91)),AVERAGE(F91:F92),IF(OR(ISTEXT(F91),ISTEXT(F92)),\"Invalid\",AVERAGE(F91:F92))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C93),ISBLANK(C94)),\"\",IF(AVERAGE($C93:$C94)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C93:$C94)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C93),ISBLANK(C94)),\"\",STDEV(C93,C94))","v":""},{"$f":"IF(ISTEXT(D94),\"\",D94/((C93+C94)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C93),ISBLANK(C94)),\"\",IF(OR(ISBLANK(C94),ISBLANK(C93)),AVERAGE(F93:F94),IF(OR(ISTEXT(F93),ISTEXT(F94)),\"Invalid\",AVERAGE(F93:F94))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C95),ISBLANK(C96)),\"\",IF(AVERAGE($C95:$C96)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C95:$C96)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
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[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C143),ISBLANK(C144)),\"\",IF(AVERAGE($C143:$C144)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C143:$C144)<$D$25,\"GREATER than Standard 4, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C143),ISBLANK(C144)),\"\",STDEV(C143,C144))","v":""},{"$f":"IF(ISTEXT(D144),\"\",D144/((C143+C144)/2)*100)","v":""},{"$f":"IF(ISBLANK(C144),\"\",IF(C144<=$C$32,\"invalid\",IF(C144>=$C$29,\"invalid\",$C$31*(($C$29-C144)/(C144-$C$32))^(1/$C$30))))","v":""},{"$f":"IF(AND(ISBLANK(C143),ISBLANK(C144)),\"\",IF(OR(ISBLANK(C144),ISBLANK(C143)),AVERAGE(F143:F144),IF(OR(ISTEXT(F143),ISTEXT(F144)),\"Invalid\",AVERAGE(F143:F144))))","v":""}]

{"sheet":"6 Standards","index":3,"n_rows":145,"n_cols":19,"rows_kept":145,"cut":false,"hidden":false,"merged":[[135,7,136,9],[137,7,138,9],[139,7,140,9],[141,7,142,9],[143,7,144,9],[133,7,134,9],[111,7,112,9],[113,7,114,9],[115,7,116,9],[117,7,118,9],[119,7,120,9],[121,7,122,9],[123,7,124,9],[125,7,126,9],[127,7,128,9],[129,7,130,9],[131,7,132,9],[109,7,110,9],[87,7,88,9],[89,7,90,9],[91,7,92,9],[93,7,94,9],[95,7,96,9],[97,7,98,9],[99,7,100,9],[101,7,102,9],[103,7,104,9],[105,7,106,9],[107,7,108,9],[85,7,86,9],[63,7,64,9],[65,7,66,9],[67,7,68,9],[69,7,70,9],[71,7,72,9],[73,7,74,9],[75,7,76,9],[77,7,78,9],[79,7,80,9],[81,7,82,9],[83,7,84,9],[61,7,62,9],[39,7,40,9],[41,7,42,9],[43,7,44,9],[45,7,46,9],[47,7,48,9],[49,7,50,9],[51,7,52,9],[53,7,54,9],[55,7,56,9],[57,7,58,9],[59,7,60,9],[37,7,38,9],[7,15,7,24],[8,15,8,24],[9,15,9,24],[11,15,11,24],[12,15,12,24],[13,15,13,24],[14,15,14,24],[16,15,16,24],[18,1,18,7],[35,1,35,9],[36,7,36,9],[6,15,6,24],[1,1,1,5],[1,15,1,24],[3,1,3,6],[3,15,3,24],[5,15,5,24]]}
[]
[null,"** USER MUST ONLY INPUT VALUES IN GREY-COLORED CELLS **",null,null,null,null,"Kit Lot #",null,null,null,null,null,null,null,null,"Before you start:"]
[]
[null,"Enter the absorbance values of each standard into the grey boxes",null,null,null,null,null,null,null,null,null,null,null,null,null,"Check that \"solver\" is installed"]
[null,"Std 0","Std 1","Std 2","Std 3","Std 4","Std 5"]
[null,0.63900000000000001,0.499,0.442,0.34499999999999997,0.24099999999999999,0.16200000000000001,null,null,null,null,null,5.8999999999999997E-2,null,null,"Input OD values for standards (Fig 4 in protocol)"]
[null,0.56799999999999995,0.50800000000000001,0.45100000000000001,0.36299999999999999,0.24099999999999999,0.16300000000000001,null,null,null,null,null,6.0999999999999999E-2,null,null,"Select solver  (Target Cell: $e$32 - Cell Changes: $c$35:$c$38 - Optimize: Minimize - GRG Nonlinear )"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"\"Solve\""]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Check graph and parametres (A>0.7; D<0.1; "]
[]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Calculate MC TADDA-concentration in samples in wells: "]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Input average OD of unknown samples in cell c48 (sample 1) , c49 (sample 2)…."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Calculated ELISA MCTADDA concentration are given automatically in cell d48 (sample 1), c49 (sample 2),…."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Sample concentrations should only be used from datapoints that are within the valid working range of the standard curve"]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"If OD is outsite the Ic20 to Ic80 range, dilution sholud be adjusted and the samples assayed again"]
[]
[null,"Enter the standard concentrations of each standard into the grey boxes"]
[null,"Standards (ng/ml)","OD (averaged)","Predicted values","Residual Squares","B/Bo","Std Dev","%CV"]
[null,0,{"$f":"(B6+B7)/2","v":0.60349999999999993},{"$f":"($C$30-$C$33)/(1+(B21/$C$32)^$C$31)+$C$33","v":0.59940972406264748},{"$f":"(C21-D21)^2","v":1.6730357243684464E-5},null,{"$f":"STDEV(B6,B7)","v":5.0204581464244918E-2},{"$f":"(G21/C21)*100","v":8.3189033080770383}]
[null,0.15,{"$f":"(C6+C7)/2","v":0.50350000000000006},0.51464111593882733,1.241244643623911E-4,{"$f":"C22/C$21","v":0.83429991714995877},{"$f":"STDEV(C6,C7)","v":6.3639610306789338E-3},1.263944593977941,null,null,null,null,null,null,null,null,"OD","MCTADDA-conc","X=C x ((A-Y)/(Y-D))^(1/B)"]
[null,0.4,{"$f":"(D6+D7)/2","v":0.44650000000000001},0.44240530459998989,1.6766530418864018E-5,{"$f":"C23/C$21","v":0.73985086992543503},{"$f":"STDEV(D6,D7)","v":6.3639610306789329E-3},1.4252992229964017,null,null,null,null,null,null,null,"Ic20",{"$f":"$C$33+($C$30-$C$33)*20/100","v":0.11988194481252951},{"$f":"C32*((C30-Q23)/(Q23-C33))^(1/C31)","v":8.8361990885689163}]
[null,1,{"$f":"(E6+E7)/2","v":0.35399999999999998},0.34708720968364942,4.7786669957830057E-5,{"$f":"C24/C$21","v":0.58657829328914668},{"$f":"STDEV(E6,E7)","v":1.2727922061357866E-2},3.5954582094231262,null,null,null,null,null,null,null,"Ic80",{"$f":"$C$33+($C$30-$C$33)*80/100","v":0.47952777925011802},{"$f":"C32*((C30-Q24)/(Q24-C33))^(1/C31)","v":0.25564700932822509}]
[null,2.5,{"$f":"(F6+F7)/2","v":0.24099999999999999},0.240807411781606,3.7090221864171525E-8,{"$f":"C25/C$21","v":0.39933719966859987},{"$f":"STDEV(F6,F7)","v":0},0]
[null,5,{"$f":"(G6+G7)/2","v":0.16250000000000001},0.16829328639061586,3.3562167203694868E-5,{"$f":"C26/C$21","v":0.26926263463131733},{"$f":"STDEV(G6,G7)","v":7.0710678118654816E-4},0.43514263457633728]
[null,"Sum Of Squares",null,null,{"$f":"SUM(E21:E26)","v":2.3900727940832868E-4}]
[]
[null,"Parameter (Y=(A-D)/(1+(X/C)^B)+D)",null,null,"R-Squared"]
[null,"A",0.59940972406264748,"(Max.)",{"$f":"(CORREL(C21:C26,D21:D26))^2","v":0.99827750802868886}]
[null,"B",0.7825949520178026,"(Slope)"]
[null,"C",1.5029796641410134,"(IC50)"]
[null,"D",0,"(Min.)"]
[]
[]
[null,"Enter descriptions and absorbance values of each sample and control into the grey boxes"]
[null,"Sample/Control","Absorbances","Std Dev","%CV","Sample Concentration (ng/ml)","Sample Concentration Avg (ng/ml)","Comments"]
[null,"Control",0.38100000000000001,null,null,{"$f":"IF(ISBLANK(C38),\"\",IF(C38<=$C$33,\"Invalid\",IF(C38>=$C$30,\"Invalid\",$C$32*(($C$30-C38)/(C38-$C$33))^(1/$C$31))))","v":0.73819116224840164},null,{"$f":"IF(AND(ISBLANK(C38),ISBLANK(C39)),\"\",IF(AVERAGE($C38:$C39)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C38:$C39)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,"Control",0.38900000000000001,{"$f":"IF(OR(ISBLANK(C38),ISBLANK(C39)),\"\",STDEV(C38,C39))","v":5.6568542494923853E-3},{"$f":"IF(ISTEXT(D39),\"\",D39/((C38+C39)/2)*100)","v":1.4693127920759441},{"$f":"IF(ISBLANK(C39),\"\",IF(C39<=$C$33,\"Invalid\",IF(C39>=$C$30,\"Invalid\",$C$32*(($C$30-C39)/(C39-$C$33))^(1/$C$31))))","v":0.68537606503826509},{"$f":"IF(AND(ISBLANK(C38),ISBLANK(C39)),\"\",IF(OR(ISBLANK(C39),ISBLANK(C38)),AVERAGE(F38:F39),IF(OR(ISTEXT(F38),ISTEXT(F39)),\"Invalid\",AVERAGE(F38:F39))))","v":0.71178361364333331}]
[null,"LRB",0.58299999999999996,null,null,1.569054038756677E-2,null,{"$f":"IF(AND(ISBLANK(C40),ISBLANK(C41)),\"\",IF(AVERAGE($C40:$C41)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C40:$C41)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":"LESS than Standard 1, outside of quantifiable range"}]
[null,"LRB",0.623,{"$f":"IF(OR(ISBLANK(C40),ISBLANK(C41)),\"\",STDEV(C40,C41))","v":2.8284271247461926E-2},{"$f":"IF(ISTEXT(D41),\"\",D41/((C40+C41)/2)*100)","v":4.6905922466769363},"Invalid",{"$f":"IF(AND(ISBLANK(C40),ISBLANK(C41)),\"\",IF(OR(ISBLANK(C41),ISBLANK(C40)),AVERAGE(F40:F41),IF(OR(ISTEXT(F40),ISTEXT(F41)),\"Invalid\",AVERAGE(F40:F41))))","v":"Invalid"}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C42),ISBLANK(C43)),\"\",IF(AVERAGE($C42:$C43)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C42:$C43)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C42),ISBLANK(C43)),\"\",STDEV(C42,C43))","v":""},{"$f":"IF(ISTEXT(D43),\"\",D43/((C42+C43)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C42),ISBLANK(C43)),\"\",IF(OR(ISBLANK(C43),ISBLANK(C42)),AVERAGE(F42:F43),IF(OR(ISTEXT(F42),ISTEXT(F43)),\"Invalid\",AVERAGE(F42:F43))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C44),ISBLANK(C45)),\"\",IF(AVERAGE($C44:$C45)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C44:$C45)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C44),ISBLANK(C45)),\"\",STDEV(C44,C45))","v":""},{"$f":"IF(ISTEXT(D45),\"\",D45/((C44+C45)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C44),ISBLANK(C45)),\"\",IF(OR(ISBLANK(C45),ISBLANK(C44)),AVERAGE(F44:F45),IF(OR(ISTEXT(F44),ISTEXT(F45)),\"Invalid\",AVERAGE(F44:F45))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C46),ISBLANK(C47)),\"\",IF(AVERAGE($C46:$C47)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C46:$C47)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C46),ISBLANK(C47)),\"\",STDEV(C46,C47))","v":""},{"$f":"IF(ISTEXT(D47),\"\",D47/((C46+C47)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C46),ISBLANK(C47)),\"\",IF(OR(ISBLANK(C47),ISBLANK(C46)),AVERAGE(F46:F47),IF(OR(ISTEXT(F46),ISTEXT(F47)),\"Invalid\",AVERAGE(F46:F47))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C48),ISBLANK(C49)),\"\",IF(AVERAGE($C48:$C49)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C48:$C49)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C48),ISBLANK(C49)),\"\",STDEV(C48,C49))","v":""},{"$f":"IF(ISTEXT(D49),\"\",D49/((C48+C49)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C48),ISBLANK(C49)),\"\",IF(OR(ISBLANK(C49),ISBLANK(C48)),AVERAGE(F48:F49),IF(OR(ISTEXT(F48),ISTEXT(F49)),\"Invalid\",AVERAGE(F48:F49))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C50),ISBLANK(C51)),\"\",IF(AVERAGE($C50:$C51)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C50:$C51)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C50),ISBLANK(C51)),\"\",STDEV(C50,C51))","v":""},{"$f":"IF(ISTEXT(D51),\"\",D51/((C50+C51)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C50),ISBLANK(C51)),\"\",IF(OR(ISBLANK(C51),ISBLANK(C50)),AVERAGE(F50:F51),IF(OR(ISTEXT(F50),ISTEXT(F51)),\"Invalid\",AVERAGE(F50:F51))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C52),ISBLANK(C53)),\"\",IF(AVERAGE($C52:$C53)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C52:$C53)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C52),ISBLANK(C53)),\"\",STDEV(C52,C53))","v":""},{"$f":"IF(ISTEXT(D53),\"\",D53/((C52+C53)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C52),ISBLANK(C53)),\"\",IF(OR(ISBLANK(C53),ISBLANK(C52)),AVERAGE(F52:F53),IF(OR(ISTEXT(F52),ISTEXT(F53)),\"Invalid\",AVERAGE(F52:F53))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C54),\"\",IF(C54<=$C$33,\"Invalid\",IF(C54>=$C$30,\"Invalid\",$C$32*(($C$30-C54)/(C54-$C$33))^(1/$C$31))))","v":""},null,{"$f":"IF(AND(ISBLANK(C54),ISBLANK(C55)),\"\",IF(AVERAGE($C54:$C55)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C54:$C55)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C54),ISBLANK(C55)),\"\",STDEV(C54,C55))","v":""},{"$f":"IF(ISTEXT(D55),\"\",D55/((C54+C55)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C54),ISBLANK(C55)),\"\",IF(OR(ISBLANK(C55),ISBLANK(C54)),AVERAGE(F54:F55),IF(OR(ISTEXT(F54),ISTEXT(F55)),\"Invalid\",AVERAGE(F54:F55))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C56),ISBLANK(C57)),\"\",IF(AVERAGE($C56:$C57)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C56:$C57)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C56),ISBLANK(C57)),\"\",STDEV(C56,C57))","v":""},{"$f":"IF(ISTEXT(D57),\"\",D57/((C56+C57)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C56),ISBLANK(C57)),\"\",IF(OR(ISBLANK(C57),ISBLANK(C56)),AVERAGE(F56:F57),IF(OR(ISTEXT(F56),ISTEXT(F57)),\"Invalid\",AVERAGE(F56:F57))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C58),\"\",IF(C58<=$C$33,\"Invalid\",IF(C58>=$C$30,\"Invalid\",$C$32*(($C$30-C58)/(C58-$C$33))^(1/$C$31))))","v":""},null,{"$f":"IF(AND(ISBLANK(C58),ISBLANK(C59)),\"\",IF(AVERAGE($C58:$C59)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C58:$C59)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C58),ISBLANK(C59)),\"\",STDEV(C58,C59))","v":""},{"$f":"IF(ISTEXT(D59),\"\",D59/((C58+C59)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C58),ISBLANK(C59)),\"\",IF(OR(ISBLANK(C59),ISBLANK(C58)),AVERAGE(F58:F59),IF(OR(ISTEXT(F58),ISTEXT(F59)),\"Invalid\",AVERAGE(F58:F59))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C60),ISBLANK(C61)),\"\",IF(AVERAGE($C60:$C61)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C60:$C61)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C60),ISBLANK(C61)),\"\",STDEV(C60,C61))","v":""},{"$f":"IF(ISTEXT(D61),\"\",D61/((C60+C61)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C60),ISBLANK(C61)),\"\",IF(OR(ISBLANK(C61),ISBLANK(C60)),AVERAGE(F60:F61),IF(OR(ISTEXT(F60),ISTEXT(F61)),\"Invalid\",AVERAGE(F60:F61))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C62),ISBLANK(C63)),\"\",IF(AVERAGE($C62:$C63)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C62:$C63)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C62),ISBLANK(C63)),\"\",STDEV(C62,C63))","v":""},{"$f":"IF(ISTEXT(D63),\"\",D63/((C62+C63)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C62),ISBLANK(C63)),\"\",IF(OR(ISBLANK(C63),ISBLANK(C62)),AVERAGE(F62:F63),IF(OR(ISTEXT(F62),ISTEXT(F63)),\"Invalid\",AVERAGE(F62:F63))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C64),ISBLANK(C65)),\"\",IF(AVERAGE($C64:$C65)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C64:$C65)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C64),ISBLANK(C65)),\"\",STDEV(C64,C65))","v":""},{"$f":"IF(ISTEXT(D65),\"\",D65/((C64+C65)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C64),ISBLANK(C65)),\"\",IF(OR(ISBLANK(C65),ISBLANK(C64)),AVERAGE(F64:F65),IF(OR(ISTEXT(F64),ISTEXT(F65)),\"Invalid\",AVERAGE(F64:F65))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C66),ISBLANK(C67)),\"\",IF(AVERAGE($C66:$C67)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C66:$C67)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C66),ISBLANK(C67)),\"\",STDEV(C66,C67))","v":""},{"$f":"IF(ISTEXT(D67),\"\",D67/((C66+C67)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C66),ISBLANK(C67)),\"\",IF(OR(ISBLANK(C67),ISBLANK(C66)),AVERAGE(F66:F67),IF(OR(ISTEXT(F66),ISTEXT(F67)),\"Invalid\",AVERAGE(F66:F67))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C68),ISBLANK(C69)),\"\",IF(AVERAGE($C68:$C69)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C68:$C69)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C68),ISBLANK(C69)),\"\",STDEV(C68,C69))","v":""},{"$f":"IF(ISTEXT(D69),\"\",D69/((C68+C69)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C68),ISBLANK(C69)),\"\",IF(OR(ISBLANK(C69),ISBLANK(C68)),AVERAGE(F68:F69),IF(OR(ISTEXT(F68),ISTEXT(F69)),\"Invalid\",AVERAGE(F68:F69))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C70),ISBLANK(C71)),\"\",IF(AVERAGE($C70:$C71)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C70:$C71)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C70),ISBLANK(C71)),\"\",STDEV(C70,C71))","v":""},{"$f":"IF(ISTEXT(D71),\"\",D71/((C70+C71)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C70),ISBLANK(C71)),\"\",IF(OR(ISBLANK(C71),ISBLANK(C70)),AVERAGE(F70:F71),IF(OR(ISTEXT(F70),ISTEXT(F71)),\"Invalid\",AVERAGE(F70:F71))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C72),ISBLANK(C73)),\"\",IF(AVERAGE($C72:$C73)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C72:$C73)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
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[null,null,null,null,null,{"$f":"IF(ISBLANK(C122),\"\",IF(C122<=$C$33,\"Invalid\",IF(C122>=$C$30,\"Invalid\",$C$32*(($C$30-C122)/(C122-$C$33))^(1/$C$31))))","v":""},null,{"$f":"IF(AND(ISBLANK(C122),ISBLANK(C123)),\"\",IF(AVERAGE($C122:$C123)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C122:$C123)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C122),ISBLANK(C123)),\"\",STDEV(C122,C123))","v":""},{"$f":"IF(ISTEXT(D123),\"\",D123/((C122+C123)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C122),ISBLANK(C123)),\"\",IF(OR(ISBLANK(C123),ISBLANK(C122)),AVERAGE(F122:F123),IF(OR(ISTEXT(F122),ISTEXT(F123)),\"Invalid\",AVERAGE(F122:F123))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C124),ISBLANK(C125)),\"\",IF(AVERAGE($C124:$C125)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C124:$C125)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C124),ISBLANK(C125)),\"\",STDEV(C124,C125))","v":""},{"$f":"IF(ISTEXT(D125),\"\",D125/((C124+C125)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C124),ISBLANK(C125)),\"\",IF(OR(ISBLANK(C125),ISBLANK(C124)),AVERAGE(F124:F125),IF(OR(ISTEXT(F124),ISTEXT(F125)),\"Invalid\",AVERAGE(F124:F125))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C126),\"\",IF(C126<=$C$33,\"Invalid\",IF(C126>=$C$30,\"Invalid\",$C$32*(($C$30-C126)/(C126-$C$33))^(1/$C$31))))","v":""},null,{"$f":"IF(AND(ISBLANK(C126),ISBLANK(C127)),\"\",IF(AVERAGE($C126:$C127)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C126:$C127)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C126),ISBLANK(C127)),\"\",STDEV(C126,C127))","v":""},{"$f":"IF(ISTEXT(D127),\"\",D127/((C126+C127)/2)*100)","v":""},{"$f":"IF(ISBLANK(C127),\"\",IF(C127<=$C$33,\"Invalid\",IF(C127>=$C$30,\"Invalid\",$C$32*(($C$30-C127)/(C127-$C$33))^(1/$C$31))))","v":""},{"$f":"IF(AND(ISBLANK(C126),ISBLANK(C127)),\"\",IF(OR(ISBLANK(C127),ISBLANK(C126)),AVERAGE(F126:F127),IF(OR(ISTEXT(F126),ISTEXT(F127)),\"Invalid\",AVERAGE(F126:F127))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C128),ISBLANK(C129)),\"\",IF(AVERAGE($C128:$C129)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C128:$C129)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C128),ISBLANK(C129)),\"\",STDEV(C128,C129))","v":""},{"$f":"IF(ISTEXT(D129),\"\",D129/((C128+C129)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C128),ISBLANK(C129)),\"\",IF(OR(ISBLANK(C129),ISBLANK(C128)),AVERAGE(F128:F129),IF(OR(ISTEXT(F128),ISTEXT(F129)),\"Invalid\",AVERAGE(F128:F129))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C130),\"\",IF(C130<=$C$33,\"Invalid\",IF(C130>=$C$30,\"Invalid\",$C$32*(($C$30-C130)/(C130-$C$33))^(1/$C$31))))","v":""},null,{"$f":"IF(AND(ISBLANK(C130),ISBLANK(C131)),\"\",IF(AVERAGE($C130:$C131)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C130:$C131)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C130),ISBLANK(C131)),\"\",STDEV(C130,C131))","v":""},{"$f":"IF(ISTEXT(D131),\"\",D131/((C130+C131)/2)*100)","v":""},{"$f":"IF(ISBLANK(C131),\"\",IF(C131<=$C$33,\"Invalid\",IF(C131>=$C$30,\"Invalid\",$C$32*(($C$30-C131)/(C131-$C$33))^(1/$C$31))))","v":""},{"$f":"IF(AND(ISBLANK(C130),ISBLANK(C131)),\"\",IF(OR(ISBLANK(C131),ISBLANK(C130)),AVERAGE(F130:F131),IF(OR(ISTEXT(F130),ISTEXT(F131)),\"Invalid\",AVERAGE(F130:F131))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C132),ISBLANK(C133)),\"\",IF(AVERAGE($C132:$C133)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C132:$C133)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C132),ISBLANK(C133)),\"\",STDEV(C132,C133))","v":""},{"$f":"IF(ISTEXT(D133),\"\",D133/((C132+C133)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C132),ISBLANK(C133)),\"\",IF(OR(ISBLANK(C133),ISBLANK(C132)),AVERAGE(F132:F133),IF(OR(ISTEXT(F132),ISTEXT(F133)),\"Invalid\",AVERAGE(F132:F133))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C134),ISBLANK(C135)),\"\",IF(AVERAGE($C134:$C135)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C134:$C135)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C134),ISBLANK(C135)),\"\",STDEV(C134,C135))","v":""},{"$f":"IF(ISTEXT(D135),\"\",D135/((C134+C135)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C134),ISBLANK(C135)),\"\",IF(OR(ISBLANK(C135),ISBLANK(C134)),AVERAGE(F134:F135),IF(OR(ISTEXT(F134),ISTEXT(F135)),\"Invalid\",AVERAGE(F134:F135))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C136),ISBLANK(C137)),\"\",IF(AVERAGE($C136:$C137)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C136:$C137)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C136),ISBLANK(C137)),\"\",STDEV(C136,C137))","v":""},{"$f":"IF(ISTEXT(D137),\"\",D137/((C136+C137)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C136),ISBLANK(C137)),\"\",IF(OR(ISBLANK(C137),ISBLANK(C136)),AVERAGE(F136:F137),IF(OR(ISTEXT(F136),ISTEXT(F137)),\"Invalid\",AVERAGE(F136:F137))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C138),ISBLANK(C139)),\"\",IF(AVERAGE($C138:$C139)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C138:$C139)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C138),ISBLANK(C139)),\"\",STDEV(C138,C139))","v":""},{"$f":"IF(ISTEXT(D139),\"\",D139/((C138+C139)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C138),ISBLANK(C139)),\"\",IF(OR(ISBLANK(C139),ISBLANK(C138)),AVERAGE(F138:F139),IF(OR(ISTEXT(F138),ISTEXT(F139)),\"Invalid\",AVERAGE(F138:F139))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C140),ISBLANK(C141)),\"\",IF(AVERAGE($C140:$C141)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C140:$C141)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C140),ISBLANK(C141)),\"\",STDEV(C140,C141))","v":""},{"$f":"IF(ISTEXT(D141),\"\",D141/((C140+C141)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C140),ISBLANK(C141)),\"\",IF(OR(ISBLANK(C141),ISBLANK(C140)),AVERAGE(F140:F141),IF(OR(ISTEXT(F140),ISTEXT(F141)),\"Invalid\",AVERAGE(F140:F141))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C142),ISBLANK(C143)),\"\",IF(AVERAGE($C142:$C143)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C142:$C143)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C142),ISBLANK(C143)),\"\",STDEV(C142,C143))","v":""},{"$f":"IF(ISTEXT(D143),\"\",D143/((C142+C143)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C142),ISBLANK(C143)),\"\",IF(OR(ISBLANK(C143),ISBLANK(C142)),AVERAGE(F142:F143),IF(OR(ISTEXT(F142),ISTEXT(F143)),\"Invalid\",AVERAGE(F142:F143))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C144),ISBLANK(C145)),\"\",IF(AVERAGE($C144:$C145)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C144:$C145)<$D$26,\"GREATER than Standard 5, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C144),ISBLANK(C145)),\"\",STDEV(C144,C145))","v":""},{"$f":"IF(ISTEXT(D145),\"\",D145/((C144+C145)/2)*100)","v":""},{"$f":"IF(ISBLANK(C145),\"\",IF(C145<=$C$33,\"invalid\",IF(C145>=$C$30,\"invalid\",$C$32*(($C$30-C145)/(C145-$C$33))^(1/$C$31))))","v":""},{"$f":"IF(AND(ISBLANK(C144),ISBLANK(C145)),\"\",IF(OR(ISBLANK(C145),ISBLANK(C144)),AVERAGE(F144:F145),IF(OR(ISTEXT(F144),ISTEXT(F145)),\"Invalid\",AVERAGE(F144:F145))))","v":""}]

{"sheet":"7 Standards","index":4,"n_rows":146,"n_cols":19,"rows_kept":146,"cut":false,"hidden":false,"merged":[[136,7,137,9],[138,7,139,9],[140,7,141,9],[142,7,143,9],[144,7,145,9],[102,7,103,9],[104,7,105,9],[106,7,107,9],[108,7,109,9],[98,7,99,9],[90,7,91,9],[92,7,93,9],[94,7,95,9],[96,7,97,9],[100,7,101,9],[82,7,83,9],[84,7,85,9],[132,7,133,9],[134,7,135,9],[112,7,113,9],[114,7,115,9],[116,7,117,9],[118,7,119,9],[120,7,121,9],[122,7,123,9],[124,7,125,9],[126,7,127,9],[128,7,129,9],[130,7,131,9],[110,7,111,9],[88,7,89,9],[86,7,87,9],[74,7,75,9],[52,7,53,9],[54,7,55,9],[56,7,57,9],[58,7,59,9],[60,7,61,9],[62,7,63,9],[64,7,65,9],[66,7,67,9],[68,7,69,9],[70,7,71,9],[72,7,73,9],[76,7,77,9],[78,7,79,9],[80,7,81,9],[50,7,51,9],[14,15,14,24],[16,15,16,24],[18,1,18,7],[36,1,36,9],[37,7,37,9],[38,7,39,9],[40,7,41,9],[42,7,43,9],[44,7,45,9],[46,7,47,9],[48,7,49,9],[13,15,13,24],[1,1,1,5],[1,15,1,24],[3,15,3,24],[5,15,5,24],[6,15,6,24],[7,15,7,24],[8,15,8,24],[9,15,9,24],[11,15,11,24],[12,15,12,24],[3,1,3,7]]}
[]
[null,"** USER MUST ONLY INPUT VALUES IN GREY-COLORED CELLS **",null,null,null,null,"Kit Lot #",null,null,null,null,null,null,null,null,"Before you start:"]
[]
[null,"Enter the absorbance values of each standard into the grey boxes",null,null,null,null,null,null,null,null,null,null,null,null,null,"Check that \"solver\" is installed"]
[null,"Std 0","Std 1","Std 2","Std 3","Std 4","Std 5","Std 6"]
[null,1.407,1.2,1.1240000000000001,0.77900000000000003,0.58199999999999996,0.38800000000000001,0.38700000000000001,null,null,null,null,5.8999999999999997E-2,null,null,"Input OD values for standards (Fig 4 in protocol)"]
[null,1.3440000000000001,1.26,1.141,0.83699999999999997,0.55300000000000005,0.52200000000000002,0.35399999999999998,null,null,null,null,6.0999999999999999E-2,null,null,"Select solver  (Target Cell: $e$32 - Cell Changes: $c$35:$c$38 - Optimize: Minimize - GRG Nonlinear )"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"\"Solve\""]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Check graph and parametres (A>0.7; D<0.1; "]
[]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Calculate MC TADDA-concentration in samples in wells: "]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Input average OD of unknown samples in cell c48 (sample 1) , c49 (sample 2)…."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Calculated ELISA MCTADDA concentration are given automatically in cell d48 (sample 1), c49 (sample 2),…."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Sample concentrations should only be used from datapoints that are within the valid working range of the standard curve"]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"If OD is outsite the Ic20 to Ic80 range, dilution sholud be adjusted and the samples assayed again"]
[]
[null,"Enter the standard concentrations of each standard into the grey boxes"]
[null,"Standards (ng/ml)","OD (averaged)","Predicted values","Residual Squares","B/Bo","Std Dev","%CV"]
[null,0,{"$f":"(B6+B7)/2","v":1.3755000000000002},{"$f":"($C$31-$C$34)/(1+(B21/$C$33)^$C$32)+$C$34","v":1.3673243178303898},{"$f":"(C21-D21)^2","v":6.6841778938485401E-5},null,{"$f":"STDEV(B6,B7)","v":4.4547727214752454E-2},{"$f":"(G21/C21)*100","v":3.2386570130681531}]
[null,0.05,{"$f":"(C6+C7)/2","v":1.23},1.2537339041896853,5.6329820808516385E-4,{"$f":"C22/C$21","v":0.89422028353326055},{"$f":"STDEV(C6,C7)","v":4.2426406871192889E-2},3.4493013716416985,null,null,null,null,null,null,null,null,"OD","MCTADDA-conc","X=C x ((A-Y)/(Y-D))^(1/B)"]
[null,0.1,{"$f":"(D6+D7)/2","v":1.1325000000000001},1.1129372927942094,3.8269951321949437E-4,0.82333696837513626,{"$f":"STDEV(D6,D7)","v":1.2020815280171239E-2},1.0614406428407275,null,null,null,null,null,null,null,"Ic20",{"$f":"$C$34+($C$31-$C$34)*20/100","v":0.53606455885603421},{"$f":"C33*((C31-Q23)/(Q23-C34))^(1/C32)","v":0.60084729292152617}]
[null,0.25,{"$f":"(E6+E7)/2","v":0.80800000000000005},0.80694604878767029,1.1108131579713757E-6,0.58742275536168664,{"$f":"STDEV(E6,E7)","v":4.1012193308819715E-2},5.0757664986163009,null,null,null,null,null,null,null,"Ic80",{"$f":"$C$34+($C$31-$C$34)*80/100","v":1.1595093780868009},{"$f":"C33*((C31-Q24)/(Q24-C34))^(1/C32)","v":8.3073086738776336E-2}]
[null,0.5,{"$f":"(F6+F7)/2","v":0.5675},0.58217480315706194,2.1534984769851489E-4,0.4125772446383133,{"$f":"STDEV(F6,F7)","v":2.0506096654409819E-2},3.6134091020986463]
[null,1,{"$f":"(G6+G7)/2","v":0.45500000000000002},0.44159688516357015,1.7964348731852633E-4,0.33078880407124678,{"$f":"STDEV(G6,G7)","v":9.4752308678997296E-2},20.824683226153251]
[null,2,{"$f":"(H6+H7)/2","v":0.3705},0.37428268276185317,1.4308688876821151E-5,0.26935659760087238,{"$f":"STDEV(H6,H7)","v":2.333452377915609E-2},6.2981170793943564]
[null,"Sum Of Squares",null,null,{"$f":"SUM(E21:E27)","v":1.4232523372949774E-3}]
[]
[null,"Parameter (Y=(A-D)/(1+(X/C)^B)+D)",null,null,"R-Squared"]
[null,"A",1.3673243178303898,"(Max.)",{"$f":"(CORREL(C21:C27,D21:D27))^2","v":0.99852947729518005}]
[null,"B",1.4012739583751284,"(Slope)"]
[null,"C",0.22341494865301403,"(IC50)"]
[null,"D",0.32824961911244532,"(Min.)"]
[]
[]
[null,"Enter descriptions and absorbance values of each sample and control into the grey boxes"]
[null,"Sample/Control","Absorbances","Std Dev","%CV","Sample Concentration (ng/ml)","Sample Concentration Avg (ng/ml)","Comments"]
[null,"Control",0.47,null,null,{"$f":"IF(ISBLANK(C39),\"\",IF(C39<=$C$34,\"Invalid\",IF(C39>=$C$31,\"Invalid\",$C$33*(($C$31-C39)/(C39-$C$34))^(1/$C$32))))","v":0.83375483618932367},null,{"$f":"IF(AND(ISBLANK(C39),ISBLANK(C40)),\"\",IF(AVERAGE($C39:$C40)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C39:$C40)<$D$27,\"GREATER than Standard 6, outside of quantifiable range\",\"\")))","v":""}]
[null,"Control",0.52500000000000002,{"$f":"IF(OR(ISBLANK(C39),ISBLANK(C40)),\"\",STDEV(C39,C40))","v":3.889087296526015E-2},{"$f":"IF(ISTEXT(D40),\"\",D40/((C39+C40)/2)*100)","v":7.817260897539728},{"$f":"IF(ISBLANK(C40),\"\",IF(C40<=$C$34,\"Invalid\",IF(C40>=$C$31,\"Invalid\",$C$33*(($C$31-C40)/(C40-$C$34))^(1/$C$32))))","v":0.63069453507236339},{"$f":"IF(AND(ISBLANK(C39),ISBLANK(C40)),\"\",IF(OR(ISBLANK(C40),ISBLANK(C39)),AVERAGE(F39:F40),IF(OR(ISTEXT(F39),ISTEXT(F40)),\"Invalid\",AVERAGE(F39:F40))))","v":0.73222468563084353}]
[null,"blank",1.343,null,null,1.5588131042940956E-2,null,{"$f":"IF(AND(ISBLANK(C41),ISBLANK(C42)),\"\",IF(AVERAGE($C41:$C42)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C41:$C42)<$D$27,\"GREATER than Standard 6, outside of quantifiable range\",\"\")))","v":"LESS than Standard 1, outside of quantifiable range"}]
[null,"blank",1.3120000000000001,{"$f":"IF(OR(ISBLANK(C41),ISBLANK(C42)),\"\",STDEV(C41,C42))","v":2.1920310216782913E-2},{"$f":"IF(ISTEXT(D42),\"\",D42/((C41+C42)/2)*100)","v":1.6512474739572816},2.8647653355703254E-2,{"$f":"IF(AND(ISBLANK(C41),ISBLANK(C42)),\"\",IF(OR(ISBLANK(C42),ISBLANK(C41)),AVERAGE(F41:F42),IF(OR(ISTEXT(F41),ISTEXT(F42)),\"Invalid\",AVERAGE(F41:F42))))","v":2.2117892199322104E-2}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C43),ISBLANK(C44)),\"\",IF(AVERAGE($C43:$C44)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C43:$C44)<$D$27,\"GREATER than Standard 6, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C43),ISBLANK(C44)),\"\",STDEV(C43,C44))","v":""},{"$f":"IF(ISTEXT(D44),\"\",D44/((C43+C44)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C43),ISBLANK(C44)),\"\",IF(OR(ISBLANK(C44),ISBLANK(C43)),AVERAGE(F43:F44),IF(OR(ISTEXT(F43),ISTEXT(F44)),\"Invalid\",AVERAGE(F43:F44))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C45),ISBLANK(C46)),\"\",IF(AVERAGE($C45:$C46)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C45:$C46)<$D$27,\"GREATER than Standard 6, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C45),ISBLANK(C46)),\"\",STDEV(C45,C46))","v":""},{"$f":"IF(ISTEXT(D46),\"\",D46/((C45+C46)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C45),ISBLANK(C46)),\"\",IF(OR(ISBLANK(C46),ISBLANK(C45)),AVERAGE(F45:F46),IF(OR(ISTEXT(F45),ISTEXT(F46)),\"Invalid\",AVERAGE(F45:F46))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C47),ISBLANK(C48)),\"\",IF(AVERAGE($C47:$C48)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C47:$C48)<$D$27,\"GREATER than Standard 6, outside of quantifiable range\",\"\")))","v":""}]
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[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C145),ISBLANK(C146)),\"\",IF(AVERAGE($C145:$C146)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C145:$C146)<$D$27,\"GREATER than Standard 6, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C145),ISBLANK(C146)),\"\",STDEV(C145,C146))","v":""},{"$f":"IF(ISTEXT(D146),\"\",D146/((C145+C146)/2)*100)","v":""},{"$f":"IF(ISBLANK(C146),\"\",IF(C146<=$C$34,\"invalid\",IF(C146>=$C$31,\"invalid\",$C$33*(($C$31-C146)/(C146-$C$34))^(1/$C$32))))","v":""},{"$f":"IF(AND(ISBLANK(C145),ISBLANK(C146)),\"\",IF(OR(ISBLANK(C146),ISBLANK(C145)),AVERAGE(F145:F146),IF(OR(ISTEXT(F145),ISTEXT(F146)),\"Invalid\",AVERAGE(F145:F146))))","v":""}]

{"sheet":"8 Standards","index":5,"n_rows":147,"n_cols":19,"rows_kept":147,"cut":false,"hidden":false,"merged":[[137,7,138,9],[139,7,140,9],[141,7,142,9],[143,7,144,9],[145,7,146,9],[103,7,104,9],[105,7,106,9],[107,7,108,9],[109,7,110,9],[99,7,100,9],[91,7,92,9],[93,7,94,9],[95,7,96,9],[97,7,98,9],[101,7,102,9],[83,7,84,9],[85,7,86,9],[133,7,134,9],[135,7,136,9],[113,7,114,9],[115,7,116,9],[117,7,118,9],[119,7,120,9],[121,7,122,9],[123,7,124,9],[125,7,126,9],[127,7,128,9],[129,7,130,9],[131,7,132,9],[111,7,112,9],[89,7,90,9],[87,7,88,9],[75,7,76,9],[53,7,54,9],[55,7,56,9],[57,7,58,9],[59,7,60,9],[61,7,62,9],[63,7,64,9],[65,7,66,9],[67,7,68,9],[69,7,70,9],[71,7,72,9],[73,7,74,9],[77,7,78,9],[79,7,80,9],[81,7,82,9],[51,7,52,9],[14,15,14,24],[16,15,16,24],[18,1,18,7],[37,1,37,9],[38,7,38,9],[39,7,40,9],[41,7,42,9],[43,7,44,9],[45,7,46,9],[47,7,48,9],[49,7,50,9],[13,15,13,24],[1,1,1,5],[1,15,1,24],[3,15,3,24],[5,15,5,24],[6,15,6,24],[7,15,7,24],[8,15,8,24],[9,15,9,24],[11,15,11,24],[12,15,12,24],[3,1,3,8]]}
[]
[null,"** USER MUST ONLY INPUT VALUES IN GREY-COLORED CELLS **",null,null,null,null,"Kit Lot #",null,null,null,null,null,null,null,null,"Before you start:"]
[]
[null,"Enter the absorbance values of each standard into the grey boxes",null,null,null,null,null,null,null,null,null,null,null,null,null,"Check that \"solver\" is installed"]
[null,"Std 0","Std 1","Std 2","Std 3","Std 4","Std 5","Std 6","Std 7"]
[null,1.85,1.55,1.25,0.95,0.75,0.55000000000000004,0.35,0.15,null,null,null,5.8999999999999997E-2,null,null,"Input OD values for standards (Fig 4 in protocol)"]
[null,1.83,1.53,1.23,0.93,0.73,0.53,0.33,0.13,null,null,null,6.0999999999999999E-2,null,null,"Select solver  (Target Cell: $e$32 - Cell Changes: $c$35:$c$38 - Optimize: Minimize - GRG Nonlinear )"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"\"Solve\""]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Check graph and parametres (A>0.7; D<0.1; "]
[]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Calculate MC TADDA-concentration in samples in wells: "]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Input average OD of unknown samples in cell c48 (sample 1) , c49 (sample 2)…."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Calculated ELISA MCTADDA concentration are given automatically in cell d48 (sample 1), c49 (sample 2),…."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Sample concentrations should only be used from datapoints that are within the valid working range of the standard curve"]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"If OD is outsite the Ic20 to Ic80 range, dilution sholud be adjusted and the samples assayed again"]
[]
[null,"Enter the standard concentrations of each standard into the grey boxes"]
[null,"Standards (ng/ml)","OD (averaged)","Predicted values","Residual Squares","B/Bo","Std Dev","%CV"]
[null,0,{"$f":"(B6+B7)/2","v":1.84},{"$f":"($C$32-$C$35)/(1+(B21/$C$34)^$C$33)+$C$35","v":1.8472783492660785},{"$f":"(C21-D21)^2","v":5.2974368039023965E-5},null,{"$f":"STDEV(B6,B7)","v":1.4142135623730963E-2},{"$f":"(G21/C21)*100","v":0.76859432737668276}]
[null,0.15,{"$f":"(C6+C7)/2","v":1.54},1.5048648870491532,1.2344761620687636E-3,{"$f":"C22/C$21","v":0.83695652173913038},{"$f":"STDEV(C6,C7)","v":1.4142135623730963E-2},0.91832049504746505,null,null,null,null,null,null,null,null,"OD","MCTADDA-conc","X=C x ((A-Y)/(Y-D))^(1/B)"]
[null,0.4,{"$f":"(D6+D7)/2","v":1.24},1.2625815433279657,5.0992609907279181E-4,0.67391304347826086,{"$f":"STDEV(D6,D7)","v":1.4142135623730963E-2},1.1404948083654003,null,null,null,null,null,null,null,"Ic20",{"$f":"$C$35+($C$32-$C$35)*20/100","v":0.36945566985321571},{"$f":"C34*((C32-Q23)/(Q23-C35))^(1/C33)","v":7.8434445131485173}]
[null,1,{"$f":"(E6+E7)/2","v":0.94},0.97252779948250689,1.0580577391741792E-3,0.51086956521739124,{"$f":"STDEV(E6,E7)","v":1.4142135623730885E-2},1.5044825131628601,null,null,null,null,null,null,null,"Ic80",{"$f":"$C$35+($C$32-$C$35)*80/100","v":1.4778226794128628},{"$f":"C34*((C32-Q24)/(Q24-C35))^(1/C33)","v":0.17081458411124611}]
[null,2,{"$f":"(F6+F7)/2","v":0.74},0.74300895513806076,9.0538110228622761E-6,0.40217391304347822,{"$f":"STDEV(F6,F7)","v":1.4142135623730963E-2},1.9110994086122923]
[null,5,{"$f":"(G6+G7)/2","v":0.54},0.47528987353638369,4.1874004669372206E-3,0.29347826086956524,{"$f":"STDEV(G6,G7)","v":1.4142135623730963E-2},2.6189140043946226]
[null,10,{"$f":"(H6+H7)/2","v":0.33999999999999997},0.32016910164799361,3.9326452944760831E-4,0.18478260869565216,{"$f":"STDEV(H6,H7)","v":1.4142135623730925E-2},4.1594516540385076]
[null,20,{"$f":"(I6+I7)/2","v":0.14000000000000001},0.20799995357911116,4.623993686761271E-3,7.6086956521739135E-2,{"$f":"STDEV(I6,I7)","v":1.4142135623730944E-2},10.101525445522102]
[null,"Sum Of Squares",null,null,{"$f":"SUM(E21:E28)","v":1.206914686252372E-2}]
[]
[null,"Parameter (Y=(A-D)/(1+(X/C)^B)+D)",null,null,"R-Squared"]
[null,"A",1.8472783492660785,"(Max.)",{"$f":"(CORREL(C21:C28,D21:D28))^2","v":0.9951201257575526}]
[null,"B",0.72450848993219852,"(Slope)"]
[null,"C",1.1574863768153385,"(IC50)"]
[null,"D",0,"(Min.)"]
[]
[]
[null,"Enter descriptions and absorbance values of each sample and control into the grey boxes"]
[null,"Sample/Control","Absorbances","Std Dev","%CV","Sample Concentration (ng/ml)","Sample Concentration Avg (ng/ml)","Comments"]
[null,"Control",1.03,null,null,{"$f":"IF(ISBLANK(C40),\"\",IF(C40<=$C$35,\"Invalid\",IF(C40>=$C$32,\"Invalid\",$C$34*(($C$32-C40)/(C40-$C$35))^(1/$C$33))))","v":0.84109758771196441},null,{"$f":"IF(AND(ISBLANK(C40),ISBLANK(C41)),\"\",IF(AVERAGE($C40:$C41)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C40:$C41)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,"Control",1.05,{"$f":"IF(OR(ISBLANK(C40),ISBLANK(C41)),\"\",STDEV(C40,C41))","v":1.4142135623730963E-2},{"$f":"IF(ISTEXT(D41),\"\",D41/((C40+C41)/2)*100)","v":1.3598207330510541},{"$f":"IF(ISBLANK(C41),\"\",IF(C41<=$C$35,\"Invalid\",IF(C41>=$C$32,\"Invalid\",$C$34*(($C$32-C41)/(C41-$C$35))^(1/$C$33))))","v":0.79152928849276449},{"$f":"IF(AND(ISBLANK(C40),ISBLANK(C41)),\"\",IF(OR(ISBLANK(C41),ISBLANK(C40)),AVERAGE(F40:F41),IF(OR(ISTEXT(F40),ISTEXT(F41)),\"Invalid\",AVERAGE(F40:F41))))","v":0.81631343810236445}]
[null,"Sample 1",1.23,null,null,0.4469274144076672,null,{"$f":"IF(AND(ISBLANK(C42),ISBLANK(C43)),\"\",IF(AVERAGE($C42:$C43)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C42:$C43)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,"Sample 1",1.25,{"$f":"IF(OR(ISBLANK(C42),ISBLANK(C43)),\"\",STDEV(C42,C43))","v":1.4142135623730963E-2},{"$f":"IF(ISTEXT(D43),\"\",D43/((C42+C43)/2)*100)","v":1.1404948083654003},0.41766212273422654,{"$f":"IF(AND(ISBLANK(C42),ISBLANK(C43)),\"\",IF(OR(ISBLANK(C43),ISBLANK(C42)),AVERAGE(F42:F43),IF(OR(ISTEXT(F42),ISTEXT(F43)),\"Invalid\",AVERAGE(F42:F43))))","v":0.43229476857094684}]
[null,"Sample 2",0.85,null,null,1.4431167480977698,null,{"$f":"IF(AND(ISBLANK(C44),ISBLANK(C45)),\"\",IF(AVERAGE($C44:$C45)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C44:$C45)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,"Sample 2",0.83,{"$f":"IF(OR(ISBLANK(C44),ISBLANK(C45)),\"\",STDEV(C44,C45))","v":1.4142135623730963E-2},{"$f":"IF(ISTEXT(D45),\"\",D45/((C44+C45)/2)*100)","v":1.6835875742536861},1.5327692901522605,{"$f":"IF(AND(ISBLANK(C44),ISBLANK(C45)),\"\",IF(OR(ISBLANK(C45),ISBLANK(C44)),AVERAGE(F44:F45),IF(OR(ISTEXT(F44),ISTEXT(F45)),\"Invalid\",AVERAGE(F44:F45))))","v":1.4879430191250151}]
[null,"Sample 3",1.63,null,null,7.170746030646559E-2,null,{"$f":"IF(AND(ISBLANK(C46),ISBLANK(C47)),\"\",IF(AVERAGE($C46:$C47)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C46:$C47)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":"LESS than Standard 1, outside of quantifiable range"}]
[null,"Sample 3",1.65,{"$f":"IF(OR(ISBLANK(C46),ISBLANK(C47)),\"\",STDEV(C46,C47))","v":1.4142135623730963E-2},{"$f":"IF(ISTEXT(D47),\"\",D47/((C46+C47)/2)*100)","v":0.86232534291042462},6.1712149104721142E-2,{"$f":"IF(AND(ISBLANK(C46),ISBLANK(C47)),\"\",IF(OR(ISBLANK(C47),ISBLANK(C46)),AVERAGE(F46:F47),IF(OR(ISTEXT(F46),ISTEXT(F47)),\"Invalid\",AVERAGE(F46:F47))))","v":6.6709804705593373E-2}]
[null,"Sample 4",0.13,null,null,40.797170740284585,null,{"$f":"IF(AND(ISBLANK(C48),ISBLANK(C49)),\"\",IF(AVERAGE($C48:$C49)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C48:$C49)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":"GREATER than Standard 7, outside of quantifiable range"}]
[null,"Sample 4",0.12,{"$f":"IF(OR(ISBLANK(C48),ISBLANK(C49)),\"\",STDEV(C48,C49))","v":7.0710678118654814E-3},{"$f":"IF(ISTEXT(D49),\"\",D49/((C48+C49)/2)*100)","v":5.656854249492385},45.929401196424891,{"$f":"IF(AND(ISBLANK(C48),ISBLANK(C49)),\"\",IF(OR(ISBLANK(C49),ISBLANK(C48)),AVERAGE(F48:F49),IF(OR(ISTEXT(F48),ISTEXT(F49)),\"Invalid\",AVERAGE(F48:F49))))","v":43.363285968354738}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C50),ISBLANK(C51)),\"\",IF(AVERAGE($C50:$C51)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C50:$C51)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C50),ISBLANK(C51)),\"\",STDEV(C50,C51))","v":""},{"$f":"IF(ISTEXT(D51),\"\",D51/((C50+C51)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C50),ISBLANK(C51)),\"\",IF(OR(ISBLANK(C51),ISBLANK(C50)),AVERAGE(F50:F51),IF(OR(ISTEXT(F50),ISTEXT(F51)),\"Invalid\",AVERAGE(F50:F51))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C52),ISBLANK(C53)),\"\",IF(AVERAGE($C52:$C53)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C52:$C53)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C52),ISBLANK(C53)),\"\",STDEV(C52,C53))","v":""},{"$f":"IF(ISTEXT(D53),\"\",D53/((C52+C53)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C52),ISBLANK(C53)),\"\",IF(OR(ISBLANK(C53),ISBLANK(C52)),AVERAGE(F52:F53),IF(OR(ISTEXT(F52),ISTEXT(F53)),\"Invalid\",AVERAGE(F52:F53))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C54),ISBLANK(C55)),\"\",IF(AVERAGE($C54:$C55)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C54:$C55)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C54),ISBLANK(C55)),\"\",STDEV(C54,C55))","v":""},{"$f":"IF(ISTEXT(D55),\"\",D55/((C54+C55)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C54),ISBLANK(C55)),\"\",IF(OR(ISBLANK(C55),ISBLANK(C54)),AVERAGE(F54:F55),IF(OR(ISTEXT(F54),ISTEXT(F55)),\"Invalid\",AVERAGE(F54:F55))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C56),\"\",IF(C56<=$C$35,\"Invalid\",IF(C56>=$C$32,\"Invalid\",$C$34*(($C$32-C56)/(C56-$C$35))^(1/$C$33))))","v":""},null,{"$f":"IF(AND(ISBLANK(C56),ISBLANK(C57)),\"\",IF(AVERAGE($C56:$C57)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C56:$C57)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C56),ISBLANK(C57)),\"\",STDEV(C56,C57))","v":""},{"$f":"IF(ISTEXT(D57),\"\",D57/((C56+C57)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C56),ISBLANK(C57)),\"\",IF(OR(ISBLANK(C57),ISBLANK(C56)),AVERAGE(F56:F57),IF(OR(ISTEXT(F56),ISTEXT(F57)),\"Invalid\",AVERAGE(F56:F57))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C58),ISBLANK(C59)),\"\",IF(AVERAGE($C58:$C59)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C58:$C59)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C58),ISBLANK(C59)),\"\",STDEV(C58,C59))","v":""},{"$f":"IF(ISTEXT(D59),\"\",D59/((C58+C59)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C58),ISBLANK(C59)),\"\",IF(OR(ISBLANK(C59),ISBLANK(C58)),AVERAGE(F58:F59),IF(OR(ISTEXT(F58),ISTEXT(F59)),\"Invalid\",AVERAGE(F58:F59))))","v":""}]
[null,null,null,null,null,{"$f":"IF(ISBLANK(C60),\"\",IF(C60<=$C$35,\"Invalid\",IF(C60>=$C$32,\"Invalid\",$C$34*(($C$32-C60)/(C60-$C$35))^(1/$C$33))))","v":""},null,{"$f":"IF(AND(ISBLANK(C60),ISBLANK(C61)),\"\",IF(AVERAGE($C60:$C61)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C60:$C61)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C60),ISBLANK(C61)),\"\",STDEV(C60,C61))","v":""},{"$f":"IF(ISTEXT(D61),\"\",D61/((C60+C61)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C60),ISBLANK(C61)),\"\",IF(OR(ISBLANK(C61),ISBLANK(C60)),AVERAGE(F60:F61),IF(OR(ISTEXT(F60),ISTEXT(F61)),\"Invalid\",AVERAGE(F60:F61))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C62),ISBLANK(C63)),\"\",IF(AVERAGE($C62:$C63)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C62:$C63)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C62),ISBLANK(C63)),\"\",STDEV(C62,C63))","v":""},{"$f":"IF(ISTEXT(D63),\"\",D63/((C62+C63)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C62),ISBLANK(C63)),\"\",IF(OR(ISBLANK(C63),ISBLANK(C62)),AVERAGE(F62:F63),IF(OR(ISTEXT(F62),ISTEXT(F63)),\"Invalid\",AVERAGE(F62:F63))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C64),ISBLANK(C65)),\"\",IF(AVERAGE($C64:$C65)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C64:$C65)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C64),ISBLANK(C65)),\"\",STDEV(C64,C65))","v":""},{"$f":"IF(ISTEXT(D65),\"\",D65/((C64+C65)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C64),ISBLANK(C65)),\"\",IF(OR(ISBLANK(C65),ISBLANK(C64)),AVERAGE(F64:F65),IF(OR(ISTEXT(F64),ISTEXT(F65)),\"Invalid\",AVERAGE(F64:F65))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C66),ISBLANK(C67)),\"\",IF(AVERAGE($C66:$C67)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C66:$C67)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C66),ISBLANK(C67)),\"\",STDEV(C66,C67))","v":""},{"$f":"IF(ISTEXT(D67),\"\",D67/((C66+C67)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C66),ISBLANK(C67)),\"\",IF(OR(ISBLANK(C67),ISBLANK(C66)),AVERAGE(F66:F67),IF(OR(ISTEXT(F66),ISTEXT(F67)),\"Invalid\",AVERAGE(F66:F67))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C68),ISBLANK(C69)),\"\",IF(AVERAGE($C68:$C69)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C68:$C69)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C68),ISBLANK(C69)),\"\",STDEV(C68,C69))","v":""},{"$f":"IF(ISTEXT(D69),\"\",D69/((C68+C69)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C68),ISBLANK(C69)),\"\",IF(OR(ISBLANK(C69),ISBLANK(C68)),AVERAGE(F68:F69),IF(OR(ISTEXT(F68),ISTEXT(F69)),\"Invalid\",AVERAGE(F68:F69))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C70),ISBLANK(C71)),\"\",IF(AVERAGE($C70:$C71)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C70:$C71)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
[null,null,null,{"$f":"IF(OR(ISBLANK(C70),ISBLANK(C71)),\"\",STDEV(C70,C71))","v":""},{"$f":"IF(ISTEXT(D71),\"\",D71/((C70+C71)/2)*100)","v":""},"",{"$f":"IF(AND(ISBLANK(C70),ISBLANK(C71)),\"\",IF(OR(ISBLANK(C71),ISBLANK(C70)),AVERAGE(F70:F71),IF(OR(ISTEXT(F70),ISTEXT(F71)),\"Invalid\",AVERAGE(F70:F71))))","v":""}]
[null,null,null,null,null,"",null,{"$f":"IF(AND(ISBLANK(C72),ISBLANK(C73)),\"\",IF(AVERAGE($C72:$C73)>$D$22,\"LESS than Standard 1, outside of quantifiable range\",IF(AVERAGE($C72:$C73)<$D$28,\"GREATER than Standard 7, outside of quantifiable range\",\"\")))","v":""}]
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